Flint D J, Gardner M J
Hannah Research Institute, Ayr, U.K.
J Endocrinol. 1993 May;137(2):203-11. doi: 10.1677/joe.0.1370203.
Neonatal female rats were treated for 3 weeks (short term) or 8 weeks (long term) with antiserum to rat GH (anti-rGH) with or without replacement therapy with recombinant bovine GH (bGH). Body weight gain and tail length were significantly suppressed within the first 3 weeks and were even more markedly suppressed when treatment was continued for 8 weeks. When treatment was stopped in short-term-treated animals the rate of body weight gain recovered, although without evidence of catch-up growth. These effects were all normalized by concurrent treatment with bGH. Long-term anti-rGH treatment caused a profound reduction (80%) in the number of differentiated adipocytes in two internal fat depots, whilst the subcutaneous depot was only moderately affected (20%). In contrast, after recovery from short-term treatment with anti-rGH, the internal depots were only marginally decreased in both weight and adipocyte numbers, whereas the subcutaneous depot was actually doubled in size compared with controls, due entirely to an increase in the number of differentiated adipocytes. These data clearly demonstrate for the first time that GH is required for the differentiation of adipocytes in vivo. In addition, the results demonstrate distinct effects at different anatomical sites and suggest that GH may be one factor responsible for the differences described in numerous metabolic parameters and hormonal sensitivities of adipose tissue derived from different locations within the body.
新生雌性大鼠用抗大鼠生长激素（anti-rGH）治疗3周（短期）或8周（长期），同时或不同时给予重组牛生长激素（bGH）替代治疗。在最初3周内，体重增加和尾长显著受到抑制，若持续治疗8周，抑制作用则更为明显。短期治疗的动物停止治疗后，体重增加速率有所恢复，尽管没有追赶生长的迹象。同时给予bGH可使这些效应恢复正常。长期抗rGH治疗导致两个内部脂肪库中分化脂肪细胞数量大幅减少（80%），而皮下脂肪库仅受到中度影响（20%）。相反，短期抗rGH治疗恢复后，内部脂肪库的重量和脂肪细胞数量仅略有减少，而皮下脂肪库的大小实际上比对照组增加了一倍，这完全是由于分化脂肪细胞数量增加所致。这些数据首次清楚地表明，生长激素是体内脂肪细胞分化所必需的。此外，结果表明在不同解剖部位有不同效应，并提示生长激素可能是导致身体不同部位来源的脂肪组织在众多代谢参数和激素敏感性方面存在差异的一个因素。
