Seidel H J
Exp Hematol. 1977 Jan;5(1):19-26.
The hematological effects of a single dose of 50 mg/kg Methylnitrosourea (MNU),administered i.v. to CBA/J mice, are described. Special emphasis was given to the number of pluripotent (CFUS) and granulocytic committed (CFUc) stem cells found in the bone marrow and spleen. In the peripheral blood a decreased number of reticulocytes, granulocytes, lymphocytes and thrombocytes was seen. This was followed by a rapid return of reticulocytes and a very slow return of thrombocytes. The bone marrow cellularity was below normal for at least 2 weeks with a markedly reduced number of lymphocytes. A rapid decrease in bone marrow and splenic CFUS and CFUc to 20-50% of control values was observed within 3 hours in mice injected with MNU. Seven days after MNU treatment, CFUS was about 10% of normal in both tissues. Contrary to this, CFUc had undergone significant regeneration. After 3 weeks CFUS returned to normal levels. At no stage could colony stimulating activity be detected in the serum.
描述了给CBA/J小鼠静脉注射单剂量50mg/kg甲基亚硝基脲（MNU）后的血液学效应。特别强调了在骨髓和脾脏中发现的多能（CFUS）和粒细胞定向（CFUc）干细胞的数量。在外周血中，可见网织红细胞、粒细胞、淋巴细胞和血小板数量减少。随后网织红细胞迅速恢复，血小板恢复非常缓慢。骨髓细胞密度至少2周低于正常，淋巴细胞数量明显减少。在注射MNU的小鼠中，3小时内骨髓和脾脏中的CFUS和CFUc迅速降至对照值的20 - 50%。MNU治疗7天后，两个组织中的CFUS约为正常水平的10%。与此相反，CFUc已经经历了显著的再生。3周后CFUS恢复到正常水平。在任何阶段血清中都未检测到集落刺激活性。
