Salimi A, Ghadirian E
Montreal General Hospital, Research Institute, Quebec, Canada.
Microb Pathog. 1993 Apr;14(4):261-74. doi: 10.1006/mpat.1993.1026.
The present study was undertaken to determine whether recombinant tumor necrosis factor alpha (TNF) could induce Kupffer cells to kill Entamoeba histolytica parasite in vitro. C57BL/6 mice were used in this study. The liver was perfused and Kupffer cells harvested and treated with TNF for 6 h. It was found that Kupffer cells treated with TNF are able to kill amoebic trophozoites in vitro. These results further show that amoebicidal activity of TNF-activated Kupffer cells is dependent on the ratio of Kupffer cells to amoebic trophozoites. The maximum amoebicidal activity of Kupffer cells was observed with the ratio of one Kupffer cell to five amoebae. This study also shows that the optimal concentration of TNF is required in the induction of amoebicidal activity in Kupffer cells (10(5) units). It seems that both oxidative-dependent and -independent mechanisms are important for the killing of amoebae by the TNF-treated Kupffer cells. It is likely that TNF-treated Kupffer cells produce endogenous TNF or other cytotoxic molecules which are capable of mediating the parasite killing. Our results indicate that the immunologic production of TNF is important in the activation of Kupffer cells to kill amoebic trophozoites.
本研究旨在确定重组肿瘤坏死因子α（TNF）是否能在体外诱导库普弗细胞杀死溶组织内阿米巴寄生虫。本研究使用了C57BL/6小鼠。对肝脏进行灌注，收获库普弗细胞并用TNF处理6小时。发现用TNF处理的库普弗细胞能够在体外杀死阿米巴滋养体。这些结果进一步表明，TNF激活的库普弗细胞的杀阿米巴活性取决于库普弗细胞与阿米巴滋养体的比例。当库普弗细胞与阿米巴的比例为1:5时，观察到库普弗细胞的最大杀阿米巴活性。本研究还表明，诱导库普弗细胞产生杀阿米巴活性需要最佳浓度的TNF（10⁵单位）。TNF处理的库普弗细胞似乎通过氧化依赖性和非依赖性机制杀死阿米巴。TNF处理的库普弗细胞可能产生内源性TNF或其他能够介导杀死寄生虫的细胞毒性分子。我们的结果表明，TNF的免疫产生在激活库普弗细胞杀死阿米巴滋养体方面很重要。
