In vitro effect of human recombinant tumor necrosis factor on Entamoeba histolytica trophozoites.

The effect of tumor necrosis factor (TNF) on E. histolytica trophozoites was examined by using three virulent (IP: 0682:1, HM-1: IMSS, 200: NIH) and one nonvirulent (DKB) strain of E. histolytica. Various concentrations of recombinant TNF were added to E. histolytica trophozoites and the total parasite numbers and their viability were periodically assessed by microscopic observation and trypan blue staining after incubation at 37 degrees C in a nonhumidified chamber. In this study, concentrations of 10(1)-10(6) units of TNF were used. Over a concentration range of 10(4)-10(6) units, the number of trophozoites was significantly lowered in the amoebic cultures containing TNF as compared to untreated controls. It was also found that the effect of TNF was dependent on the densities of both virulent and non-virulent strains of E. histolytica trophozoites in axenic conditions. TNF has no significant affect on the growth of amoebae at the lower starting number of amoebae. The amoebae cultured at the higher density were growth-inhibited significantly in comparison with the control groups. When the growth of the virulent and nonvirulent strains of amoebae was compared in TNF treated culture, it was found that TNF has an inhibitory effect on both the virulent and nonvirulent strans of E. histolytica.