Riley D E, Campbell L A, Puolakkainen M, Krieger J N
Department of Urology, University of Washington School of Medicine, Seattle 98195.
Mol Microbiol. 1993 May;8(3):517-9. doi: 10.1111/j.1365-2958.1993.tb01595.x.
The human sexually transmitted parasite Trichomonas vaginalis is a representative of one of the three earliest evolving eukaryotic lineages. We investigated whether T. vaginalis has DNA sequences and peptides related to cell division control molecules universal among yeasts and higher eukaryotes. A T. vaginalis cell division control (CDC2/28) homologue was amplified by the polymerase chain reaction and sequenced. The absolute similarity with other CDC2/28 genes was 47%, with conservative replacement similarity of 67%. Western blots demonstrated a single T. vaginalis peptide reactive with antiserum to the PSTAIRE peptide, an expressed component of CDC2/28 genes in higher eukaryotes. Although eukaryotic, T. vaginalis has properties similar to those of bacteria and is the earliest evolving eukaryote reported to possess CDC2/28 DNA and peptide homologues. These observations suggest that the molecular origins of cell division control in eukaryotes preceded mitochondria, 28S ribosomes and regulated glycolysis.
人类性传播寄生虫阴道毛滴虫是最早进化的三个真核生物谱系之一的代表。我们研究了阴道毛滴虫是否具有与酵母和高等真核生物中普遍存在的细胞分裂控制分子相关的DNA序列和肽。通过聚合酶链反应扩增并测序了阴道毛滴虫细胞分裂控制（CDC2/28）同源物。与其他CDC2/28基因的绝对相似性为47%，保守替换相似性为67%。蛋白质印迹显示，阴道毛滴虫有一种单一肽与抗血清反应，该抗血清针对高等真核生物中CDC2/28基因的一个表达成分PSTAIRE肽。尽管阴道毛滴虫是真核生物，但其具有与细菌相似的特性，并且是据报道最早进化出拥有CDC2/28 DNA和肽同源物的真核生物。这些观察结果表明，真核生物中细胞分裂控制的分子起源早于线粒体、28S核糖体和调节糖酵解。
