Belokopytova I A, Kostyleva E I, Tomilin A N, Vorob'ev V I
Tsitologiia. 1993;35(4):61-7.
The sperm of fertile and infertile human males was treated with 0.25 n. HCl or 1% CTAB. Relative proportions of various protamine fractions in the obtained extracts were determined by scanning microdensitometry following electrophoresis of the total protamine in polyacrilamide gels. The findings were as follow. 1. The share of protamine P(2 + 3) in the sperm obtained from infertile males was lower than that of fertile males. 2. Protamine P2 in infertile males has a reduced affinity to DNA. It is suggested that in some cases the human male infertility may be due to some mutations within the P2 gene.
对可育和不育男性的精子用0.25N盐酸或1%十六烷基三甲基溴化铵进行处理。在聚丙烯酰胺凝胶中对总鱼精蛋白进行电泳后，通过扫描显微密度测定法确定所得提取物中各种鱼精蛋白组分的相对比例。结果如下：1. 从不育男性获得的精子中鱼精蛋白P(2 + 3)的比例低于可育男性。2. 不育男性的鱼精蛋白P2与DNA的亲和力降低。有人提出，在某些情况下，男性不育可能是由于P2基因内的某些突变所致。
