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不育男性精子中鱼精蛋白1/鱼精蛋白2比例异常。

Aberrant protamine 1/protamine 2 ratios in sperm of infertile human males.

作者信息

Balhorn R, Reed S, Tanphaichitr N

机构信息

Biomedical Sciences Division, Lawrence Livermore National Laboratory, California 94550.

出版信息

Experientia. 1988 Jan 15;44(1):52-5. doi: 10.1007/BF01960243.

DOI:10.1007/BF01960243
PMID:3350120
Abstract

Protamines were extracted from the sperm of fertile and infertile human males and the relative proportion of protamines 1, 2, and 3 were determined by scanning microdensitometry following electrophoresis of total protamine in polyacrylamide gels. The proportion of the three protamines was found to be similar in sperm obtained from different normal males. The distribution of protamines in sperm obtained from a select group of infertile males producing an elevated level of large sperm heads, in contrast, was different from that of the fertile males.

摘要

从有生育能力和无生育能力的男性精子中提取鱼精蛋白,并在聚丙烯酰胺凝胶中对总鱼精蛋白进行电泳后,通过扫描显微密度测定法确定鱼精蛋白1、2和3的相对比例。结果发现,从不同正常男性获得的精子中,三种鱼精蛋白的比例相似。相比之下,从一组特定的不育男性(其产生的大头精子水平升高)获得的精子中,鱼精蛋白的分布与有生育能力的男性不同。

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Biol Reprod. 1981 Dec;25(5):1041-51. doi: 10.1095/biolreprod25.5.1041.
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DNA and protein content of mouse sperm. Implications regarding sperm chromatin structure.小鼠精子的DNA和蛋白质含量。关于精子染色质结构的意义。
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DNA packaging in mouse spermatids. Synthesis of protamine variants and four transition proteins.
精子RNA数量以及PRM1、PRM2和TH2B转录水平反映精子特征和早期胚胎发育。
Asian J Androl. 2025 Jan 1;27(1):76-83. doi: 10.4103/aja202452. Epub 2024 Aug 27.
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What is known so far about bull sperm protamination: a review.关于公牛精子鱼精蛋白的现有研究综述
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Loss of Prm1 leads to defective chromatin protamination, impaired PRM2 processing, reduced sperm motility and subfertility in male mice.Prm1 的缺失导致染色质鱼精蛋白化缺陷、PRM2 加工受损、精子运动能力降低和雄性小鼠生育力下降。
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Gene Expression and Its Protein Abundance in Frozen-Thawed Spermatozoa as Potential Fertility Markers in Breeding Bulls.冻融精子中的基因表达及其蛋白质丰度作为种公牛潜在生育力标志物
Vet Sci. 2022 Mar 3;9(3):111. doi: 10.3390/vetsci9030111.
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The effects of sesame oil and different doses of estradiol on testicular structure, sperm parameters, and chromatin integrity in old mice.芝麻油和不同剂量雌二醇对老年小鼠睾丸结构、精子参数及染色质完整性的影响
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