苦参中生物碱的高效液相色谱分析
[HPLC analysis of alkaloids in Sophora flavescens Ait].
作者信息
Jin L X, Cui Y Y, Zhang G D
机构信息
Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing.
出版信息
Yao Xue Xue Bao. 1993;28(2):136-9.
In this paper, a HPLC method was developed for the determination of alkaloids in Sophora flavescens Ait. Experimental evidences indicate that a system of a LiChrosorb-NH2 column (4.0 mm x 250 mm) as stationary phase and CH3CN--H3PO4(pH2)--CH3CH2OH(80:8:10) as mobile phase can separate the five alkaloids, sophocarpine, matrine, sophoridine, oxysophcarpine and oxymatrine very well. This method is very easy and efficient and takes only 15 min for one run.
本文建立了一种用于测定苦参中生物碱的高效液相色谱法。实验证据表明，以LiChrosorb-NH2柱（4.0 mm×250 mm）为固定相，CH3CN-H3PO4（pH2）-CH3CH2OH（80:8:10）为流动相的体系能够很好地分离氧化槐果碱、苦参碱、槐定碱、氧化苦参碱和槐果碱这五种生物碱。该方法简便高效，一次运行仅需15分钟。
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