Lin C C, Lin C H
School of Pharmacy, Kaohsiung Medical College, Taiwan.
Am J Chin Med. 1993;21(1):33-44. doi: 10.1142/S0192415X93000054.
In order to isolate the main hepatoprotective component of Echinops grijisii, the crude drug was extracted with methanol and subjected to continuous extractions using n-hexane chloroform, ethyl acetate and n-butanol. The hepatoprotective studies of each fraction from the methanol extract of E. grijisii was conducted in Wistar albino rats with CC(1)4-induced liver damage. Hepatoprotective activity was evaluated in terms of the modification of serum transaminase values such as SGOT and SGPT, and histopathological changes of liver biopsy. The results indicated that the main hepatoprotective component was concentrated in n-butanol and aqueous fractions.
为了分离蓝刺头的主要保肝成分，将该生药用甲醇提取，然后依次用正己烷、氯仿、乙酸乙酯和正丁醇进行连续萃取。对蓝刺头甲醇提取物的各萃取部位进行保肝研究，实验采用CCl₄诱导Wistar白化大鼠肝损伤模型。通过检测血清转氨酶（如谷草转氨酶和谷丙转氨酶）水平的变化以及肝活检的组织病理学变化来评估保肝活性。结果表明，主要保肝成分集中在正丁醇部位和水部位。
