Tzortzatou-Stathopoulou F, Panagiotou J P, Xaidara A, Karydis K, Sfakianos G, Evagellinos B
First Department of Pediatrics, University of Athens, Greece.
Am J Pediatr Hematol Oncol. 1993 Aug;15(3):316-9.
Dihydrofolate reductase is an enzyme involved in cell proliferation and differentiation processes. A cytochemical method was used to detect and quantitate this enzyme at the cellular level in brain tumors in children.
Twenty-six children, aged 1-12 years, with primary brain tumors were studied, eight with medulloblastoma, 10 with glioma, and eight with ependymoma or other tumors. The cytochemical technique was applied on touch preparations performed in the operating room form biopsy specimens.
Enzyme activity was apparent as cytoplasmic granules sometimes overlying the nucleus of tumor cells.
Activity of dihydrofolate reductase in the children with medulloblastomas and high-grade gliomas was higher than that reported in leukemic blast cells. In the other brain tumors, low grade gliomas, and ependymomas, the enzyme activity was weaker.
二氢叶酸还原酶是一种参与细胞增殖和分化过程的酶。采用细胞化学方法在细胞水平检测和定量儿童脑肿瘤中的这种酶。
对26例1 - 12岁患有原发性脑肿瘤的儿童进行研究，其中8例为髓母细胞瘤，10例为胶质瘤，8例为室管膜瘤或其他肿瘤。细胞化学技术应用于在手术室从活检标本制作的触片上。
酶活性表现为细胞质颗粒，有时覆盖肿瘤细胞核。
髓母细胞瘤和高级别胶质瘤患儿中二氢叶酸还原酶的活性高于白血病原始细胞中的报道。在其他脑肿瘤、低级别胶质瘤和室管膜瘤中，酶活性较弱。
