Scott N W, Cooper J I, Edwards M L
Institute of Virology and Environmental Microbiology, Oxford, U.K.
Arch Virol. 1993;131(1-2):209-15. doi: 10.1007/BF01379093.
The coat protein gene of RNA-2 of cherry leaf roll nepovirus (CLRV) birch isolate I2 was cloned, identified, and sequenced. Transcripts derived from cDNA to the coat protein gene made a polypeptide of M(r) 51.5 k when translated in vitro. The predicted amino acid sequence of the coat protein showed little identity with nepoviruses having small RNA-2s. It did, however, have 27% sequence identity with the coat protein of tomato ringspot nepovirus which, like CLRV, has a relatively large RNA-2.
克隆、鉴定并测序了樱桃卷叶线虫传多面体病毒（CLRV）桦树分离株I2的RNA - 2外壳蛋白基因。从外壳蛋白基因的cDNA转录而来的转录本在体外翻译时产生了一个分子量为51.5k的多肽。外壳蛋白的预测氨基酸序列与具有小RNA - 2的线虫传多面体病毒几乎没有同源性。然而，它与番茄环斑线虫传多面体病毒的外壳蛋白有27%的序列同源性，番茄环斑线虫传多面体病毒与CLRV一样，具有相对较大的RNA - 2。
