Blanchflower W J, Hewitt S A, Cannavan A, Elliott C T, Kennedy D G
Veterinary Sciences Division, Department of Agriculture, Stormont, Belfast, UK.
Biol Mass Spectrom. 1993 Jun;22(6):326-30. doi: 10.1002/bms.1200220603.
A gas chromatographic/mass spectrometric method is described for the detection of clenbuterol residues in liver, muscle, urine and retina. Tissue samples are first digested using protease and any clenbuterol present is extracted using a simple liquid/liquid extraction procedure. The dried extracts are then derivatized using methylboronic acid and the derivates are subjected to gas chromatography/mass spectrometry on a magnetic sector instrument. The detection limit of the assay is 0.05 ng g-1 clenbuterol in liver, muscle or urine a 10 g sample size, and 4 ng g-1 in retina using a 0.5 g sample size. The assay is made very specific by using selected ion monitoring of three ions at a resolution of 3500 and by ion ratio measurements. The precision and reproducibility of the assay are enhanced by the use of a deuterated internal standard, with a typical coefficient of variation of 3%.
描述了一种气相色谱/质谱法，用于检测肝脏、肌肉、尿液和视网膜中的克伦特罗残留。首先使用蛋白酶消化组织样品，然后采用简单的液/液萃取程序提取其中存在的任何克伦特罗。干燥的提取物随后用甲基硼酸进行衍生化，所得衍生物在磁扇形仪器上进行气相色谱/质谱分析。该检测方法的检测限为：对于肝脏、肌肉或尿液，采用10 g样品量时为0.05 ng g-1克伦特罗；对于视网膜，采用0.5 g样品量时为4 ng g-1。通过在3500的分辨率下对三种离子进行选择离子监测以及通过离子比率测量，使该检测方法具有很高的特异性。通过使用氘代内标物提高了检测方法的精密度和重现性，典型变异系数为3%。
