Kingston R, Ordsmith N, Williams E, Thieme D
VG Organic, Manchester, UK.
Rapid Commun Mass Spectrom. 1995;9(14):1395-9. doi: 10.1002/rcm.1290091414.
A method has been developed using reliable GC derivatization techniques interfaced with a high resolution mass spectrometer. The method has proved successful for the detection of low levels (less than 1 ppm) of clenbuterol in complex biological matrices. Selected ion recording of two characteristic isotopic fragment ions provides a specific mode of detection by verifying the GC retention time of these ions and also by comparing their relative abundance. Analysis of urine samples demands higher mass spectrometric resolution, and 40 000 (10% valley) was found to be a prerequisite for accurate integration of the drug-related chromatographic peaks. The method developed is suitable for adaptation to a completely unattended automated routine incorporating sample injection, storage and retrieval of source tuning parameters, and data processing.
已开发出一种方法，该方法采用可靠的气相色谱衍生化技术，并与高分辨率质谱仪联用。事实证明，该方法可成功检测复杂生物基质中低水平（低于1 ppm）的克伦特罗。通过验证这些离子的气相色谱保留时间并比较它们的相对丰度，对两个特征性同位素碎片离子进行选择离子记录，提供了一种特定的检测模式。尿液样本分析需要更高的质谱分辨率，发现40000（10%谷宽）是准确积分与药物相关色谱峰的前提条件。所开发的方法适用于完全无人值守的自动化常规操作，包括进样、源调谐参数的存储和检索以及数据处理。
