Ibbotson S H, Tate G M, Davies J A
Academic Unit of Medicine, General Infirmary, Leeds, UK.
Blood Coagul Fibrinolysis. 1993 Jun;4(3):415-9.
Increased plasma FVIII:C concentrations occur in several conditions, including diabetes, but whether this leads to clinically relevant hypercoagulability is uncertain. Accordingly, we investigated the effects of increasing FVIII:C levels on coagulation in vitro using a computer-assisted measurement of thrombin activity. Defibrinated plasma was activated with kaolin, thrombin activity measured using the chromogenic substrate S2238 and time to generate 50% maximal thrombin activity (T50) recorded in seconds. Increasing FVIII:C levels from 100 to 350% significantly reduced T50 (mean +/- SD) from 91 +/- 3 to 64 +/- 6.2 s (P < 0.001, n = 6), and T50 correlated inversely with FVIII:C (r = -0.884, P < 0.001, n = 36). Increasing FV concentrations resulted in an additive effect with high FVIII:C levels on the rate of thrombin generation. The results showed that increasing plasma FVIII:C and FV concentrations accelerate rate of generation of thrombin activity independently, and in an additive manner.
包括糖尿病在内的多种情况下，血浆FVIII:C浓度都会升高，但这是否会导致具有临床意义的高凝状态尚不确定。因此，我们使用计算机辅助测量凝血酶活性的方法，在体外研究了升高FVIII:C水平对凝血的影响。用高岭土激活去纤维蛋白血浆，使用发色底物S2238测量凝血酶活性，并记录产生50%最大凝血酶活性所需的时间（T50），以秒为单位。将FVIII:C水平从100%提高到350%，显著缩短了T50（平均值±标准差），从91±3秒降至64±6.2秒（P<0.001，n=6），且T50与FVIII:C呈负相关（r=-0.884，P<0.001，n=36）。升高FV浓度会与高FVIII:C水平对凝血酶生成速率产生相加效应。结果表明，升高血浆FVIII:C和FV浓度会独立且以相加的方式加速凝血酶活性的生成速率。
