Kaneko T, Shirakami G, Nakao K, Nagata I, Nakagawa O, Hama N, Suga S, Miyamoto S, Kubo H, Hirai O
Department of Neurosurgery, Kokura Memorial Hospital, Kitakyushu, Japan.
Brain Res. 1993 May 28;612(1-2):104-9. doi: 10.1016/0006-8993(93)91649-d.
In order to investigate whether C-type natriuretic peptide (CNP) is present in human cerebrospinal fluid (CSF), we measured CNP-like immunoreactivity (-LI) in human CSF by specific radioimmunoassay (RIA) for CNP. We also measured atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) concentrations in human CSF. ANP-LI, BNP-LI, and CNP-LI concentrations of CSF collected from fifteen patients without neurological disorders were 0.20 +/- 0.13, 0.27 +/- 0.10, and 2.13 +/- 0.27 fmol/ml (mean +/- S.D.), respectively. In fifteen patients with neurological disorders, ANP-LI, BNP-LI, and CNP-LI concentrations in CSF were 0.21 +/- 0.18, 0.33 +/- 0.19, and 2.09 +/- 0.82 fmol/ml, respectively. Although ANP-LI and BNP-LI concentrations in plasma were much higher than those in CSF, CNP-LI was undetectable in plasma (less than 0.2 fmol/ml). These results demonstrate that three natriuretic peptides are present in CSF and that CNP is the major natriuretic peptide in human CSF. These results suggest that CNP in CSF is originated from and play important roles in the central nervous system.
为了研究C型利钠肽（CNP）是否存在于人体脑脊液（CSF）中，我们通过针对CNP的特异性放射免疫测定法（RIA）测量了人体CSF中的CNP样免疫反应性（-LI）。我们还测量了人体CSF中的心房利钠肽（ANP）和脑利钠肽（BNP）浓度。从15名无神经系统疾病的患者收集的CSF中，ANP-LI、BNP-LI和CNP-LI浓度分别为0.20±0.13、0.27±0.10和2.13±0.27 fmol/ml（平均值±标准差）。在15名患有神经系统疾病的患者中，CSF中的ANP-LI、BNP-LI和CNP-LI浓度分别为0.21±0.18、0.33±0.19和2.09±0.82 fmol/ml。尽管血浆中的ANP-LI和BNP-LI浓度远高于CSF中的浓度，但血浆中未检测到CNP-LI（低于0.2 fmol/ml）。这些结果表明，三种利钠肽存在于CSF中，且CNP是人体CSF中的主要利钠肽。这些结果表明，CSF中的CNP源自中枢神经系统并在其中发挥重要作用。
