Riggs J E, Dejneka N
Department of Biology, Rider College, Lawrenceville, New Jersey 08648.
Cell Immunol. 1993 Jul;149(2):357-63. doi: 10.1006/cimm.1993.1161.
Severe combined immune-defective (SCID) mice reconstituted with immunoglobulin heavy chain-disparate spleen (SP) and peritoneal cavity (PerC) B cells exhibit serologic dominance of IgM bearing the PerC allotype. Immunization fails to elicit IgM production from donor SP B cells although flow cytometric analyses indicate the presence of these cells in the spleen of (SP + PerC)-->SCID chimeras. This observation suggests that donor SP B cell function is absent or inhibited in the SCID chimera. In this report, ELISAspot and proliferation assays were employed to further investigate the functional properties of B cells in these mice. Plasma cells derived from the donor SP were present in the spleen of SCID recipients at a lower number than those derived from donor PerC B cells. Spleen cells isolated from SCID recipients could be activated with insolubilized MAbs directed against IgM or IgD allotypes expressed by either the SP or PerC B cell donor. In contrast, the peritoneal cavity of SCID chimeras lacked B cells responsive to MAbs specific for the donor SP IgM and IgD allotypes. These results demonstrate that B cells derived from the SP donor are functional in (SP + PerC)-->SCID chimeras.
用免疫球蛋白重链不同的脾脏（SP）和腹腔（PerC）B细胞重建的严重联合免疫缺陷（SCID）小鼠表现出携带PerC同种异型的IgM的血清学优势。尽管流式细胞术分析表明在（SP + PerC）→SCID嵌合体的脾脏中存在这些细胞，但免疫接种未能引发供体SP B细胞产生IgM。这一观察结果表明，在SCID嵌合体中供体SP B细胞功能缺失或受到抑制。在本报告中，采用酶联免疫斑点法（ELISAspot）和增殖试验进一步研究这些小鼠中B细胞的功能特性。来自供体SP的浆细胞在SCID受体的脾脏中的数量低于来自供体PerC B细胞的浆细胞。从SCID受体分离的脾细胞可用针对由SP或PerC B细胞供体表达的IgM或IgD同种异型的不溶性单克隆抗体激活。相反，SCID嵌合体的腹腔缺乏对供体SP IgM和IgD同种异型特异性单克隆抗体有反应的B细胞。这些结果表明，来自SP供体的B细胞在（SP + PerC）→SCID嵌合体中具有功能。
