Stoermann Britta, Kretschmer Karsten, Düber Sandra, Weiss Siegfried
Molecular Immunology, HZI, Helmholtz Centre for Infection Research, Braunschweig, Germany.
Eur J Immunol. 2007 Jun;37(6):1613-20. doi: 10.1002/eji.200636640.
B-1a cells are found mainly in the peritoneal cavity of mice but are also present in the spleen. Gene expression profiling defined many genes differentially expressed in B-1a cells from these two sites. To see whether this gene expression pattern was imprinted by the particular microenvironment, peritoneal or spleen cells from recombinant L2 mice mainly consisting of B-1a cells were adoptively transferred into Rag1-/- mice. Re-isolated peritoneal and splenic B-1a cells were analyzed for expression of three indicator genes--vcam-1, adamdec1 and spi-c. The expression of these genes was up-regulated in splenic and down-regulated in peritoneal cells. This particular pattern was observed for peritoneal or splenic donor cells transferred either intraperitoneally or intravenously. Similar results were obtained when levels of surface IgM or frequencies of Mac-1+ B-1 cells were compared after transfer. This suggests that the environment induces the particular genetic program of B-1a cells and argues against an independent ontogeny.
B-1a细胞主要存在于小鼠的腹腔中，但脾脏中也有。基因表达谱分析确定了许多在来自这两个部位的B-1a细胞中差异表达的基因。为了探究这种基因表达模式是否由特定的微环境所印记，将主要由B-1a细胞组成的重组L2小鼠的腹腔或脾脏细胞过继转移到Rag1-/-小鼠体内。对重新分离的腹腔和脾脏B-1a细胞进行三种指示基因——vcam-1、adamdec1和spi-c表达的分析。这些基因的表达在脾脏细胞中上调，而在腹腔细胞中下调。无论是经腹腔还是静脉注射转移的腹腔或脾脏供体细胞，均观察到这种特定模式。转移后比较表面IgM水平或Mac-1+B-1细胞频率时也获得了类似结果。这表明环境诱导了B-1a细胞的特定遗传程序，反对其独立个体发生。
