Livingston J N, Gurny P A, Lockwood D H
J Biol Chem. 1977 Jan 25;252(2):560-2.
Significant amounts of H2O2 were produced when the polyamines, spermine, or spermidine were incubated with a Krebs-Ringer phosphate buffer that contained bovine serum albumin (Fraction V). This effect was specific for certain amines and could be prevented by treatment of the albumin fraction with isoniazid or aminoguanidine. These features suggest that H2O2 is formed during oxidative deamination of the polyamines catalized by spermine oxidase, a known contaminant of Fraction V bovine serum albumin. The insulin-like effects elicited by polyamines in fat cells (e.g. enhancement of glucose transport and inhibition of cAMP-mediated lipolysis) were dependent on H2O2 production. Incubation of cells with catalase or treatment of the albumin fraction with isoniazid abolished the stimulation of glucose uptake by polyamines but did not alter the stimulatory effects of insulin or vitamin K5. The H2O2 generating activity was partially separated from the albumin by gel filtration; only those fractions which formed H2O2 provided support for the activation of glucose transport by polyamines. Also, the time needed to activate glucose uptake was markedly shortened by incubation of the albumin buffer with the polyamines before addition of the cells. These findings indicate that the polyamines do not themselves mimic the actions of insulin but that the insulin-like effects result from the formation of H2O2 which has been shown to stimulate glucose transport.
当将多胺、精胺或亚精胺与含有牛血清白蛋白（第V组分）的磷酸 Krebs - Ringer 缓冲液一起孵育时，会产生大量的过氧化氢（H₂O₂）。这种效应对某些胺具有特异性，并且可以通过用异烟肼或氨基胍处理白蛋白组分来预防。这些特征表明，H₂O₂ 是在精胺氧化酶催化的多胺氧化脱氨过程中形成的，精胺氧化酶是牛血清白蛋白第V组分中一种已知的污染物。多胺在脂肪细胞中引发的胰岛素样效应（例如增强葡萄糖转运和抑制 cAMP 介导的脂肪分解）依赖于 H₂O₂ 的产生。用过氧化氢酶孵育细胞或用异烟肼处理白蛋白组分可消除多胺对葡萄糖摄取的刺激作用，但不会改变胰岛素或维生素 K₅ 的刺激作用。通过凝胶过滤将产生 H₂O₂ 的活性与白蛋白部分分离；只有那些形成 H₂O₂ 的组分才能支持多胺对葡萄糖转运的激活。此外，在添加细胞之前，将白蛋白缓冲液与多胺一起孵育可显著缩短激活葡萄糖摄取所需的时间。这些发现表明，多胺本身并不模拟胰岛素的作用，而是胰岛素样效应是由已被证明能刺激葡萄糖转运的 H₂O₂ 的形成所导致的。
