Leu F J, Chen C F, Sun A M
Department of Pathology, Tri-Service General Hospital, Taipei, Taiwan, R.O.C.
Lab Invest. 1993 Jul;69(1):121-30.
The routine method of tissue processing or dehydration usually causes shrinkage and/or distortion. More noticeable changes are seen in large specimens rich on hydrated tissues, such as embryos. Most likely this is due to the step-wise concentration change of the solvents.
A new method of gradual solvent or medium exchange for tissue processing was developed. By using a peristaltic pump, pure solvent or embedding medium was added slowly to the processing chamber and the overflowing processed solution was slowly drained off. A computer spreadsheet program was used to calculate the concentration changes. After adding four to five times the processing volume of the solvent, the concentration of the adding solvent in the processing chamber could reach 98.15 to 99.32%. After reaching a desired endpoint, pure solvent could be used to replace the processing solution.
We compared microencapsulated pancreatic islets and liver tissue processed traditionally and with the new method. By using this design, a smooth linear and gradual change of the concentration of the processing solutions could be obtained. No shrinkage or distortion of tissues due to the dehydration artifact was noticed, and the tissue seemed to be ideally suited for accurate quantitative histologic measurements.
A very simple but efficient method for gradual solvent and medium exchange had been designed. This could efficiently prevent the shrinkage and distortion commonly produced by traditional tissue processing methods and prepare the tissue for quantitative histologic and pathologic studies.
组织处理或脱水的常规方法通常会导致组织收缩和/或变形。在富含水合组织的大标本中，如胚胎，这种变化更为明显。这很可能是由于溶剂浓度的逐步变化所致。
开发了一种用于组织处理的逐步溶剂或介质交换的新方法。通过使用蠕动泵，将纯溶剂或包埋介质缓慢添加到处理腔室中，并缓慢排出溢出的处理溶液。使用计算机电子表格程序计算浓度变化。在添加四倍至五倍处理体积的溶剂后，处理腔室中添加溶剂的浓度可达到98.15%至99.32%。达到所需终点后，可用纯溶剂替换处理溶液。
我们比较了传统方法和新方法处理的微囊化胰岛和肝组织。通过这种设计，可以获得处理溶液浓度的平滑线性和逐步变化。未观察到因脱水伪像导致的组织收缩或变形，并且该组织似乎非常适合进行精确的定量组织学测量。
设计了一种非常简单但有效的逐步溶剂和介质交换方法。这可以有效防止传统组织处理方法通常产生的收缩和变形，并为定量组织学和病理学研究准备组织。
