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一种用于协助处理大量用于光学或电子显微镜检查的组织块的简单装置。

A simple device to aid tissue processing of large numbers of blocks for light or electron microscopy.

作者信息

Smith K J, Larramendi P C

机构信息

Department of Anatomy and Cell Biology, Eastern Virginia Medical School, Norfolk 23501.

出版信息

J Electron Microsc Tech. 1988 Feb;8(2):223-4. doi: 10.1002/jemt.1060080210.

DOI:10.1002/jemt.1060080210
PMID:3073198
Abstract

A simple device is described that aids the processing of large numbers of tissue blocks for light or electron microscopy. The device has a numbers of compartments into which different tissue blocks can be placed. Each compartment has a fine mesh floor, allowing the free exchange of the reagents used in tissue processing, and a gentle flow of the reagents is promoted by a magnetic stirring bar, or a rotary mixer. All the materials used are resistant to solvents such as propylene oxide. The device is labor-saving in that many blocks can be processed as easily as a single block. The device may also be of value in quantitative microscopy, since it ensures that all tissue blocks examined experience an identical processing history.

摘要

描述了一种简单的装置,可辅助处理大量用于光学或电子显微镜检查的组织块。该装置有多个隔室,不同的组织块可放置其中。每个隔室都有一个细网底部,允许组织处理中使用的试剂自由交换,并且通过磁力搅拌棒或旋转混合器促进试剂的平缓流动。所有使用的材料都耐环氧丙烷等溶剂。该装置节省人力,因为处理多个组织块与处理单个组织块一样容易。该装置在定量显微镜检查中也可能有价值,因为它确保所有检查的组织块都经历相同的处理过程。

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