Smith K J, Larramendi P C
Department of Anatomy and Cell Biology, Eastern Virginia Medical School, Norfolk 23501.
J Electron Microsc Tech. 1988 Feb;8(2):223-4. doi: 10.1002/jemt.1060080210.
A simple device is described that aids the processing of large numbers of tissue blocks for light or electron microscopy. The device has a numbers of compartments into which different tissue blocks can be placed. Each compartment has a fine mesh floor, allowing the free exchange of the reagents used in tissue processing, and a gentle flow of the reagents is promoted by a magnetic stirring bar, or a rotary mixer. All the materials used are resistant to solvents such as propylene oxide. The device is labor-saving in that many blocks can be processed as easily as a single block. The device may also be of value in quantitative microscopy, since it ensures that all tissue blocks examined experience an identical processing history.
描述了一种简单的装置,可辅助处理大量用于光学或电子显微镜检查的组织块。该装置有多个隔室,不同的组织块可放置其中。每个隔室都有一个细网底部,允许组织处理中使用的试剂自由交换,并且通过磁力搅拌棒或旋转混合器促进试剂的平缓流动。所有使用的材料都耐环氧丙烷等溶剂。该装置节省人力,因为处理多个组织块与处理单个组织块一样容易。该装置在定量显微镜检查中也可能有价值,因为它确保所有检查的组织块都经历相同的处理过程。