Pessac B, Alliot F, Girard A
J Cell Physiol. 1977 Jan;90(1):15-22. doi: 10.1002/jcp.1040900104.
A quantitative assay for intercellular adhesion, which is a modification of the collecting aggregate assay (Roth et al., '71) is described. The use of mechanically dissociated single cells labeled with 3H-leucine, and of a gyratory shaker increased considerably the efficiency of cell collection. With chick embryo neural retina, it was shown that isotypic cell adhesion occurs in simple synthetic media and even in sodium chloride solution suggesting that divalent cations do not play a major role in adhesion of these cells. Cell collection in media without serum was not affected by metabolic or protein synthesis inhibitors. However, cell adhesion was temperature-dependent since no collection occurred at 4 degrees C but was maximal at 38 degrees C.
本文描述了一种细胞间黏附的定量检测方法，该方法是对收集聚集体检测法（Roth等人，1971年）的改进。使用用³H-亮氨酸标记的机械解离单细胞以及回转式振荡器，大大提高了细胞收集效率。对于鸡胚神经视网膜，研究表明同型细胞黏附在简单合成培养基中甚至在氯化钠溶液中都会发生，这表明二价阳离子在这些细胞的黏附中并不起主要作用。无血清培养基中的细胞收集不受代谢或蛋白质合成抑制剂的影响。然而，细胞黏附是温度依赖性的，因为在4℃时不发生收集，但在38℃时达到最大值。
