用于在薄层板上检测腺嘌呤、鸟嘌呤、尿嘧啶、胞嘧啶及其烷基化碱基、核苷酸和核苷的检测试剂。
Detection reagent for adenine, guanine, uracil, cytosine and their alkylated bases, nucleotides and nucleosides on thin-layer plates.
作者信息
Issaq H J, Barr E W
出版信息
J Chromatogr. 1977 Feb 1;132(1):121-7. doi: 10.1016/s0021-9673(00)93778-6.
PMID:833219
Abstract
The detection of alkylated bases at the nanogram level is reported. The reaction is based on two steps, first, chlorination of the bases and, secondly, spraying the chlorinated product with o-tolidine solution to give a dark blue color. The detection of nucleotides and nucleosides is also possible. The optimum conditions and the possible mechanism of the reaction are discussed.
摘要
已报道了纳克级烷基化碱基的检测方法。该反应基于两个步骤，首先是碱基的氯化，其次是用邻联甲苯胺溶液喷洒氯化产物以产生深蓝色。核苷酸和核苷的检测也是可行的。讨论了该反应的最佳条件和可能的反应机理。
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