Shooter K V, Edwards P A, Lawley P D
Biochem J. 1971 Dec;125(3):829-40. doi: 10.1042/bj1250829.
Bacteriophage mu2 is inactivated by both mono- and di-functional sulphur mustards at relatively low extents of alkylation. No degradation of alkylated RNA was detected. Cross-linking of RNA to protein was observed with the difunctional agent, but this reaction was only a minor contribution to the inactivation. Analyses of the reaction products in bacteriophage RNA showed that, at the mean lethal doses, more than one mono-alkylation of guanine had occurred but the sum total of other types of RNA alkylation was close to a single event. The results therefore suggest that inactivation results from the mono-alkylation of adenine or cytosine. In experiments with the difunctional agent cross-linking of RNA bases or of RNA to protein also prevented replication, the existence of these reactions accounting for the greater sensitivity of the bacteriophage to this agent.
噬菌体mu2在相对较低的烷基化程度下会被单功能和双功能硫芥灭活。未检测到烷基化RNA的降解。使用双功能试剂时观察到RNA与蛋白质发生交联，但该反应对灭活的贡献较小。对噬菌体RNA中反应产物的分析表明，在平均致死剂量下，鸟嘌呤发生了不止一次单烷基化，但其他类型的RNA烷基化总数接近单次事件。因此，结果表明灭活是由腺嘌呤或胞嘧啶的单烷基化引起的。在用双功能试剂进行的实验中，RNA碱基或RNA与蛋白质的交联也会阻止复制，这些反应的存在解释了噬菌体对该试剂的更高敏感性。
