单官能和双官能硫芥对含核糖核酸的噬菌体mu2的作用。
The action of mono- and di-functional sulphur mustards on the ribonucleic acid-containing bacteriophage mu2.
作者信息
Shooter K V, Edwards P A, Lawley P D
出版信息
Biochem J. 1971 Dec;125(3):829-40. doi: 10.1042/bj1250829.
Abstract
Bacteriophage mu2 is inactivated by both mono- and di-functional sulphur mustards at relatively low extents of alkylation. No degradation of alkylated RNA was detected. Cross-linking of RNA to protein was observed with the difunctional agent, but this reaction was only a minor contribution to the inactivation. Analyses of the reaction products in bacteriophage RNA showed that, at the mean lethal doses, more than one mono-alkylation of guanine had occurred but the sum total of other types of RNA alkylation was close to a single event. The results therefore suggest that inactivation results from the mono-alkylation of adenine or cytosine. In experiments with the difunctional agent cross-linking of RNA bases or of RNA to protein also prevented replication, the existence of these reactions accounting for the greater sensitivity of the bacteriophage to this agent.
摘要
噬菌体mu2在相对较低的烷基化程度下会被单功能和双功能硫芥灭活。未检测到烷基化RNA的降解。使用双功能试剂时观察到RNA与蛋白质发生交联，但该反应对灭活的贡献较小。对噬菌体RNA中反应产物的分析表明，在平均致死剂量下，鸟嘌呤发生了不止一次单烷基化，但其他类型的RNA烷基化总数接近单次事件。因此，结果表明灭活是由腺嘌呤或胞嘧啶的单烷基化引起的。在用双功能试剂进行的实验中，RNA碱基或RNA与蛋白质的交联也会阻止复制，这些反应的存在解释了噬菌体对该试剂的更高敏感性。
相似文献
1
The action of mono- and di-functional sulphur mustards on the ribonucleic acid-containing bacteriophage mu2.单官能和双官能硫芥对含核糖核酸的噬菌体mu2的作用。
Biochem J. 1971 Dec;125(3):829-40. doi: 10.1042/bj1250829.
2
Inactivation of bacteriophage T7 by mono- and difunctional sulphur mustards in relation to cross-linking and depurination of bacteriophage DNA.单功能和双功能硫芥对噬菌体T7的灭活作用与噬菌体DNA的交联和脱嘌呤作用的关系
J Mol Biol. 1969 Jan 14;39(1):181-98. doi: 10.1016/0022-2836(69)90341-6.
3
Cytotoxicity of alkylating agents towards sensitive and resistant strains of Escherichia coli in relation to extent and mode of alkylation of cellular macromolecules and repair of alkylation lesions in deoxyribonucleic acids.烷化剂对大肠杆菌敏感菌株和耐药菌株的细胞毒性与细胞大分子的烷基化程度和方式以及脱氧核糖核酸中烷基化损伤的修复的关系。
Biochem J. 1968 Sep;109(3):433-47. doi: 10.1042/bj1090433.
4
Detection reagent for adenine, guanine, uracil, cytosine and their alkylated bases, nucleotides and nucleosides on thin-layer plates.用于在薄层板上检测腺嘌呤、鸟嘌呤、尿嘧啶、胞嘧啶及其烷基化碱基、核苷酸和核苷的检测试剂。
J Chromatogr. 1977 Feb 1;132(1):121-7. doi: 10.1016/s0021-9673(00)93778-6.
5
Independent translation of the genes of bacteriophage f2 RNA.噬菌体f2 RNA基因的独立翻译
J Mol Biol. 1968 Mar 28;32(3):681-5. doi: 10.1016/0022-2836(68)90351-3.
6
7
Methylation of ribonucleic acid by the carcinogens dimethyl sulphate, N-methyl-N-nitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine. Comparisons of chemical analyses at the nucleoside and base levels.致癌物质硫酸二甲酯、N-甲基-N-亚硝基脲和N-甲基-N'-硝基-N-亚硝基胍对核糖核酸的甲基化作用。核苷和碱基水平化学分析的比较。
Biochem J. 1972 Jun;128(1):117-32. doi: 10.1042/bj1280117.
8
The inactivation of bacteriophage R17 by ethylating agents: the lethal lesions.噬菌体R17被乙基化剂灭活：致死损伤
Chem Biol Interact. 1975 Dec;11(6):563-73. doi: 10.1016/0009-2797(75)90031-9.
9
The molecular basis for biological inactivation of nucleic acids. The action of methylating agents on the ribonucleic acid-containing bacteriophage R17.核酸生物失活的分子基础。甲基化试剂对含核糖核酸的噬菌体R17的作用。
Biochem J. 1974 Feb;137(2):303-12. doi: 10.1042/bj1370303.
10
Nucleotide sequences in bacteriophage ribonucleic acid. The eighth hopkins memorial lecture.噬菌体核糖核酸中的核苷酸序列。第八届霍普金斯纪念讲座。
Biochem J. 1971 Oct;124(5):833-43. doi: 10.1042/bj1240833a.
引用本文的文献
1
Nitrogen mustard inhibits transcription and translation in a cell free system.氮芥在无细胞系统中抑制转录和翻译。
Nucleic Acids Res. 1995 Sep 11;23(17):3508-15. doi: 10.1093/nar/23.17.3508.
2
Nucleic acid adducts of chemical carcinogens and mutagens.化学致癌物和诱变剂的核酸加合物
Arch Toxicol. 1983 Apr;52(4):249-85. doi: 10.1007/BF00316495.
3
Alkylation of double-stranded ribonucleic acid with 2-chloroethylamines.用2-氯乙胺对双链核糖核酸进行烷基化
Experientia. 1973 Nov 15;29(11):1344-6. doi: 10.1007/BF01922808.
4
Methylation of ribonucleic acid by the carcinogens dimethyl sulphate, N-methyl-N-nitrosourea and N-methyl-N'-nitro-N-nitrosoguanidine. Comparisons of chemical analyses at the nucleoside and base levels.致癌物质硫酸二甲酯、N-甲基-N-亚硝基脲和N-甲基-N'-硝基-N-亚硝基胍对核糖核酸的甲基化作用。核苷和碱基水平化学分析的比较。
Biochem J. 1972 Jun;128(1):117-32. doi: 10.1042/bj1280117.
5
The reaction of alkylating agents with bacteriophage R17. Biological effects of phosphotriester formation.烷基化剂与噬菌体R17的反应。磷酸三酯形成的生物学效应。
Biochem J. 1974 Feb;137(2):313-7. doi: 10.1042/bj1370313.
6
The molecular basis for biological inactivation of nucleic acids. The action of methylating agents on the ribonucleic acid-containing bacteriophage R17.核酸生物失活的分子基础。甲基化试剂对含核糖核酸的噬菌体R17的作用。
Biochem J. 1974 Feb;137(2):303-12. doi: 10.1042/bj1370303.
7
Persistence of methylated bases in ribonucleic acid of syrian golden hamster liver after administration of dimethylnitrosamine.给予二甲基亚硝胺后叙利亚金仓鼠肝脏核糖核酸中甲基化碱基的持久性。
Biochem J. 1979 Mar 1;177(3):967-73. doi: 10.1042/bj1770967.
本文引用的文献
1
The reaction of mustard gas with nucleic acids in vitro and in vivo.芥子气在体外和体内与核酸的反应。
Biochem J. 1960 Dec;77(3):478-84. doi: 10.1042/bj0770478.
2
SOME PHYSICAL PROPERTIES OF BACTERIOPHAGE R17 AND ITS RIBONUCLEIC ACID.噬菌体R17及其核糖核酸的一些物理性质
J Mol Biol. 1964 Apr;8:496-507. doi: 10.1016/s0022-2836(64)80007-3.
3
FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES.核酸及其组成核苷酸烷基化的进一步研究
Biochem J. 1963 Oct;89(1):127-38. doi: 10.1042/bj0890127.
4
METHYLATION AND BREAKDOWN OF MICROSOMAL AND SOLUBLE RIBONUCLEIC ACID FROM RAT LIVER BY DIAZOMETHANE.重氮甲烷对大鼠肝脏微粒体和可溶性核糖核酸的甲基化作用及降解
Biochemistry. 1963 Jul-Aug;2:733-40. doi: 10.1021/bi00904a019.
5
The structure of the DNA of bacteriophage phi-X174. II. Thermal inactivation.噬菌体φ-X174的DNA结构。II. 热失活
J Mol Biol. 1962 Oct;5:420-3. doi: 10.1016/s0022-2836(62)80030-8.
6
The structure of the DNA of bacteriophage phi-X174. III. Ultracentrifugal evidence for a ring structure.噬菌体φ-X174的DNA结构。III. 环状结构的超速离心证据
J Mol Biol. 1962 Oct;5:424-34. doi: 10.1016/s0022-2836(62)80031-x.
7
Chemical modification of viral ribonucleic acid. I. Alkylating agents.病毒核糖核酸的化学修饰。I. 烷基化剂。
Biochim Biophys Acta. 1961 Apr 29;49:169-80. doi: 10.1016/0006-3002(61)90880-0.
8
The methylation of adenylyl-(3'-5')-uridine and uridylyl-(3'-5')-adenosine with diazomethane.用重氮甲烷对腺苷酰 -（3'-5'）-尿苷和尿苷酰 -（3'-5'）-腺苷进行甲基化反应。
Biochim Biophys Acta. 1967 Jul 18;142(2):536-8. doi: 10.1016/0005-2787(67)90634-x.
9
On the methylation of dinucleoside phosphates with diazomethane.
Biochim Biophys Acta. 1966 Aug 17;123(2):430-1. doi: 10.1016/0005-2787(66)90298-x.
10
Effect of NaCIO-4 on bacteriophage: release of DNA and evidence for population heterogeneity.
Virology. 1966 Apr;28(4):742-50. doi: 10.1016/0042-6822(66)90258-3.
Zotero 插件