Hancock J M
Molecular Evolution and Systematics Group and Bioinformatics Facility, Research School of Biological Sciences, Australian National University, Canberra, ACT.
Nucleic Acids Res. 1993 Jun 25;21(12):2823-30. doi: 10.1093/nar/21.12.2823.
Analysis of TBP gene sequences from a variety of species for clustering of short sequence motifs and for over- and underrepresentation of short sequence motifs suggests involvement of slippage in the recent evolution of the TBP N-terminal domains in metazoans, Acanthamoeba and wheat. AGC, GCA and CAG are overrepresented in TBP genes of other species, suggesting that opa arrays were amplified from motifs overrepresented in ancestral species. The phylogenetic distribution of recently slippage-derived sequences in TBP is similar to that observed in the large subunit ribosomal RNAs, suggesting a propensity for certain evolutionary lineages to incorporate slippage-generated motifs into protein-coding as well as ribosomal RNA genes. Because length increase appears to have taken place independently in lineages leading to vertebrates, insects and nematodes, TBP N-terminal domains in these lineages are not homologous. All gene duplications in the TBP gene family appear to have been recent events despite strong protein sequence similarity between TRF and P. falciparum TBP. The enlargement of the TBP N-terminal domain may have coincided with acquisition of new functions and may have accompanied molecular coevolution with domains of other proteins, resulting in the acquisition of new or more complex mechanisms of transcription regulation.
对来自多种物种的TBP基因序列进行分析，以确定短序列基序的聚类以及短序列基序的过度和不足表征，结果表明，滑动参与了后生动物、棘阿米巴和小麦中TBP N端结构域的近期进化。AGC、GCA和CAG在其他物种的TBP基因中过度表征，这表明opa阵列是从祖先物种中过度表征的基序扩增而来的。TBP中最近由滑动产生的序列的系统发育分布与在大亚基核糖体RNA中观察到的相似，这表明某些进化谱系倾向于将滑动产生的基序纳入蛋白质编码以及核糖体RNA基因中。由于长度增加似乎在导致脊椎动物、昆虫和线虫的谱系中独立发生，因此这些谱系中的TBP N端结构域不是同源的。尽管TRF和恶性疟原虫TBP之间存在很强的蛋白质序列相似性，但TBP基因家族中的所有基因复制似乎都是近期发生的事件。TBP N端结构域的扩大可能与新功能的获得同时发生，并且可能伴随着与其他蛋白质结构域的分子共同进化，从而导致获得新的或更复杂的转录调控机制。
