拟南芥中一种与TATA元件结合的蛋白质导致溶液中DNA弯曲。

Bending of DNA in solution caused by a protein from Arabidopsis that binds to a TATA element.

作者信息

Takeda Y, Hirokawa H, Yamazaki K

机构信息

Bioscience Center, Nagoya University, Japan.

出版信息

Biosci Biotechnol Biochem. 1994 May;58(5):916-20. doi: 10.1271/bbb.58.916.

DOI:10.1271/bbb.58.916

PMID:7764978

Abstract

The TFIID complex, which is a general transcription factor, helps to activate and regulate the transcription associated with eukaryotic promoters. A TATA element-binding protein (TBP), a central subunit of the TFIID complex, binds to TATA elements. Circular permutation analysis showed that purified recombinant TBP-1 from Arabidopsis (aTBP-1) introduced a bend in DNA around a TATA element in DNA fragments in solution. The bending was a slow process. A bend in DNA caused by a TBP-1 was seen under an electron microscope, as was an aTBP-DNA complex after immunochemical staining.

摘要

TFIID复合物是一种通用转录因子，有助于激活和调节与真核启动子相关的转录。TATA元件结合蛋白（TBP）是TFIID复合物的核心亚基，可与TATA元件结合。环状置换分析表明，从拟南芥中纯化的重组TBP-1（aTBP-1）使溶液中DNA片段的TATA元件周围的DNA发生弯曲。这种弯曲是一个缓慢的过程。在电子显微镜下可以看到由TBP-1引起的DNA弯曲，免疫化学染色后也可以看到aTBP-DNA复合物。