Remmele W, Sauer-Manthey J
Institut für Pathologie, Dr. Horst-Schmidt-Kliniken, Klinikum der Landeshauptstadt Wiesbaden, Germany.
Virchows Arch A Pathol Anat Histopathol. 1993;422(6):467-73. doi: 10.1007/BF01606455.
Cathepsin D (CD) has been introduced as a predictor of prognosis in patients with breast cancer due to its mitogenic effect and its role in tumour metastasis. Commonly, the CD content of tumours is examined by means of a biochemical method based upon the use of monoclonal antibodies, and immunohistochemical visualization of CD has not been used extensively. The present study compares the biochemical and immunohistochemical findings in 216 cases of human breast cancer. CD may occur in tumour cells and/or macrophages. Correlation of immunohistochemically determined CD content and biochemical CD content is better in tumour cells than in macrophages. The possible causes of this observation are briefly discussed. Although a statistical correlation between the biochemical and the immunohistochemical CD findings exists, the results in individual cases vary within a wide range. Hence, the results of biochemical and immunohistochemical CD assay in an individual tumour cannot be compared directly.
组织蛋白酶D（CD）因其促有丝分裂作用及其在肿瘤转移中的作用，已被引入作为乳腺癌患者预后的预测指标。通常，肿瘤的CD含量通过基于单克隆抗体使用的生化方法进行检测，而CD的免疫组织化学可视化尚未得到广泛应用。本研究比较了216例人类乳腺癌的生化和免疫组织化学结果。CD可能存在于肿瘤细胞和/或巨噬细胞中。免疫组织化学测定的CD含量与生化CD含量在肿瘤细胞中的相关性优于巨噬细胞。简要讨论了这一观察结果的可能原因。虽然生化和免疫组织化学CD结果之间存在统计学相关性，但个别病例的结果差异很大。因此，单个肿瘤中生化和免疫组织化学CD检测的结果不能直接比较。
