Sokol'nikov A A, Kodentsova V M, Isaeva V A
Vopr Med Khim. 1993 May-Jun;39(3):50-3.
Activity of transketolase in erythrocyte hemolysates, estimated by means of measurement of sedoheptulose-7-phosphate content without preinactivation of transaldolase, proved to be 3-4-fold lower as compared with the values observed after preinactivation of transaldolase. The values of thiamine diphosphate (TDP) effect did not depend on conditions of the transketolase reaction, as well as on the colorimetric procedure used for determination of sedoheptulose-7-phosphate. A high inverse correlation was found between the value of TDP effect and an excretion of thiamine with urine in adult patients and in children of 9-13 years old. Normal excretion of thiamine with urine constituted 11-12 micrograms/h in the children of this age as judged from the TDP effect-thiamine excretion in children of this age.
在不预先使转醛醇酶失活的情况下,通过测量景天庚酮糖-7-磷酸含量来估算红细胞溶血产物中转酮醇酶的活性,结果证明该活性比预先使转醛醇酶失活后观察到的值低3至4倍。二磷酸硫胺素(TDP)效应的值不取决于转酮醇酶反应的条件,也不取决于用于测定景天庚酮糖-7-磷酸的比色程序。在成年患者和9至13岁儿童中,发现TDP效应值与尿中硫胺素排泄量之间存在高度负相关。根据该年龄段儿童的TDP效应-硫胺素排泄情况判断,该年龄段儿童尿中硫胺素的正常排泄量为每小时11至12微克。