Miettinen M
Jefferson Medical College, Thomas Jefferson University, Department of Pathology and Cell Biology, Philadelphia, Pennsylvania 19107.
Ann Med. 1993 Jun;25(3):221-33. doi: 10.3109/07853899309147868.
Immunohistochemistry has become an everyday tool in diagnostic pathology to increase the accuracy in tumour typing. The most important recent developments include availability of antibodies to leukocyte CD-antigens to nearly comprehensive lymphoma typing in paraffin sections, use of certain leukocyte antigens in typing of solid tumours, e.g. neoplastic endothelial cells (CD31 and CD34) and neural and neuroendocrine cells (CD56, CD57). Also, antibodies selective to individual keratin polypeptides promise to be helpful in the subtyping of carcinomas. Antibodies to nuclear proliferation antigens have already proven helpful in the characterization of tumour cell proliferation. Technical optimization, using enzymatic and non-enzymatic antigen retrieval modalities, is also important.
免疫组织化学已成为诊断病理学中提高肿瘤分型准确性的常用工具。最近最重要的进展包括有针对白细胞CD抗原的抗体，可在石蜡切片中对淋巴瘤进行几乎全面的分型，在实体瘤分型中使用某些白细胞抗原，如肿瘤性内皮细胞（CD31和CD34）以及神经和神经内分泌细胞（CD56、CD57）。此外，对单个角蛋白多肽具有选择性的抗体有望有助于癌的亚型分类。针对核增殖抗原的抗体已被证明有助于肿瘤细胞增殖的特征描述。采用酶促和非酶促抗原修复方法的技术优化也很重要。
