Bankfalvi A, Riehemann K, Ofner D, Checci R, Morgan J M, Piffko J, Böcker W, Jasani B, Schmid K W
Gerhard-Domagk-Institut für Pathologie, Westfälische Wilhelms-Universität, Münster.
Pathologe. 1994 Dec;15(6):345-9. doi: 10.1007/s002920050064.
Wet autoclaving is a simple, reliable and time-effective method for antigen retrieval in routinely processed archival material. Both routine diagnostic (e.g., oestrogen and progesterone receptors, cytoskeletal proteins) and research antibodies (e.g. various p53 antibodies, mdm-2, bcl-2, MIB-1) are reported to demonstrate its application. We autoclaving may allow successful application of antibodies in paraffin-embedded tissues designed for use on frozen sections. The technique has the potential to reliably handle up to 200 sections at a time, without evidence of any significant damage to the sections or nuclear morphology.
