Truong H T, Daleke D L, Huestis W H
Department of Chemistry, Stanford University, CA 94305.
Biochim Biophys Acta. 1993 Jul 25;1150(1):51-6. doi: 10.1016/0005-2736(93)90120-o.
The echinocyte-to-discocyte shape recovery of metabolically depleted erythrocytes is compromised by sulfhydryl reducing agents (Truong, H.-T.N., Ferrell, J.E., Jr. and Huestis, W.H. (1986) Blood 67, 214-221). In the presence of dithiothreitol (DTT) and sugars, crenated cells recover normal discoid shape transiently, but then develop the invaginations and intracellular inclusions of stomatocytes. The stomatogenic effects of DTT were investigated in erythrocytes recovering from crenation induced by several independent mechanisms. Cells crenated by direct manipulation of the membrane bilayer (lysophosphatidylcholine incorporation) recovered discoid shape similarly in the presence and absence of the reducing agent. In contrast, resealed ghosts and cells crenated by Mg2+ depletion or Ca2+ loading did not maintain stable discoid morphology in the presence of DTT, proceeding further to form stomatocytes. Thus cell crenation by expedients that involve cellular metabolic processes develop a redox-related morphological instability that is not found in amphipath-crenated cells.
巯基还原剂会损害代谢耗竭红细胞从棘状细胞向盘状细胞形态的恢复（Truong, H.-T.N., Ferrell, J.E., Jr.和Huestis, W.H.（1986年），《血液》67卷，214 - 221页）。在二硫苏糖醇（DTT）和糖类存在的情况下，皱缩细胞会短暂恢复正常的盘状形态，但随后会出现口形细胞的内陷和细胞内包涵体。在从几种独立机制诱导的皱缩中恢复的红细胞中研究了DTT的口形细胞生成作用。通过直接操纵膜双层（掺入溶血磷脂酰胆碱）而皱缩的细胞，在有或没有还原剂的情况下，都能类似地恢复盘状形态。相反，重新封闭的血影以及因Mg2 + 耗竭或Ca2 + 加载而皱缩的细胞，在DTT存在的情况下不能维持稳定的盘状形态，而是进一步发展形成口形细胞。因此，通过涉及细胞代谢过程的手段使细胞皱缩会产生一种与氧化还原相关的形态不稳定性，而在两亲性物质诱导皱缩的细胞中则未发现这种情况。
