Truong H T, Daleke D L, Huestis W H
Department of Chemistry, Stanford University, CA 94305.
Biochim Biophys Acta. 1993 Jul 25;1150(1):57-62. doi: 10.1016/0005-2736(93)90121-f.
Metabolic depletion induces human erythrocytes to crenate, a shape change that is reversed when ATP is regenerated by nutrient supplementation. In the presence of the sulfhydryl reducing agent dithiothreitol (DTT), this shape reversal is exaggerated, proceeding beyond normal discoid morphology to stomatocytic forms. DTT-induced stomatocytosis does not correlate consistently with alterations in cell ATP, spectrin phosphorylation, or phosphoinositide metabolism (Truong, H.-T.N., Ferrell, J.E., Jr. and Huestis, W.H. (1986) Blood 67, 214-221). The effect of DTT on outer-to-inner-monolayer transport of aminophospholipids was examined by monitoring shape changes induced by dilauroylphosphatidylserine (DLPS). Stomatocytosis induced by transport of this exogenous lipid to the membrane inner monolayer is accelerated and exaggerated by DTT. The effect of DTT on DLPS translocation is reversible and temperature dependent, consistent with the intervention of reducing agents in the activity of the aminophospholipid translocator. These findings bear on the relationship between cell redox status and shape regulation.
代谢耗竭会导致人类红细胞皱缩，当通过补充营养物质使ATP再生时，这种形状变化会逆转。在巯基还原剂二硫苏糖醇（DTT）存在的情况下，这种形状逆转会被放大，超出正常的盘状形态，发展为口形细胞形态。DTT诱导的口形细胞增多与细胞ATP、血影蛋白磷酸化或磷酸肌醇代谢的改变并不始终相关（Truong, H.-T.N., Ferrell, J.E., Jr.和Huestis, W.H.（1986年）《血液》67, 214 - 221）。通过监测二月桂酰磷脂酰丝氨酸（DLPS）诱导的形状变化，研究了DTT对氨基磷脂从外单层向内单层转运的影响。DTT会加速并放大由这种外源性脂质转运到膜内单层所诱导的口形细胞增多。DTT对DLPS易位的影响是可逆的且依赖于温度，这与还原剂对氨基磷脂转运体活性的干预一致。这些发现与细胞氧化还原状态和形状调节之间的关系有关。
