Kates M, Moldoveanu N, Stewart L C
Department of Biochemistry, University of Ottawa, Canada.
Biochim Biophys Acta. 1993 Jul 21;1169(1):46-53. doi: 10.1016/0005-2760(93)90080-s.
Recent fast atom bombardment-mass spectrometry (FABMS) studies (Tsujimoto, K., Yorimitsu, S., Takahashi, T. and Ohashi, M. (1989) J. Chem. Commun. 668-670; Frederickson, H.L., De Leeuw, J.W., Tas, A.C., Van der Greef, J., LaVos, G.F. and Boon, J.J. (1989) Biomed. Environ. Mass. Spectrom. 18, 96-105; Kloppel, K.D. and Fredrickson, H.L. (1991) J. Chromatogr. 562, 369-376) have indicated that the structure of the major phospholipid of Halobacterium salinarium (formerly Halobacterium cutirubrum) is not 2,3-diphytanyl-sn-glycerol-1-phospho-3'-sn-glycerol-1'- phosphate (PGP), but the monomethylated derivative, 2,3-diphytanyl-sn-glycerol-1-phospho-3'-sn-glycerol-1'-methylphosphate (PGP-Me). We have now confirmed the structure of the major phospholipid of extremely halophilic archaebacteria as being this methylated structure (PGP-Me) by 1H- and 13C-NMR, FABMS and TLC of the native phospholipid and its product of mild acid hydrolysis PGP. The methylated structure (PGP-Me), rather than PGP itself, is also the major phospholipid in species of other genera of extreme halophiles examined so far, such as, Haloferax, Haloarcula, Halococcus, Natronobacterium and Natronococcus.
最近的快原子轰击质谱（FABMS）研究（辻本健、寄光史、高桥哲、大桥正（1989年）《化学通讯》668 - 670页；弗雷德里克森，H.L.、德·李乌，J.W.、塔斯，A.C.、范德格里夫，J.、拉沃斯，G.F.和布恩，J.J.（1989年）《生物医学与环境质谱分析》18卷，96 - 105页；克洛佩尔，K.D.和弗雷德里克森，H.L.（1991年）《色谱杂志》562卷，369 - 376页）表明，盐生盐杆菌（原红皮盐杆菌）主要磷脂的结构不是2,3 - 二植烷酰 - sn - 甘油 - 1 - 磷酸 - 3'- sn - 甘油 - 1'- 磷酸（PGP），而是单甲基化衍生物2,3 - 二植烷酰 - sn - 甘油 - 1 - 磷酸 - 3'- sn - 甘油 - 1'- 甲基磷酸（PGP - Me）。我们现在通过对天然磷脂及其温和酸水解产物PGP进行1H - 和13C - NMR、FABMS和TLC分析，证实了极端嗜盐古细菌主要磷脂的结构为这种甲基化结构（PGP - Me）。甲基化结构（PGP - Me）而非PGP本身，也是迄今为止所检测的其他极端嗜盐菌属物种中的主要磷脂，例如嗜盐富球菌属、嗜盐碱杆菌属、嗜盐球菌属、嗜盐嗜碱杆菌属和嗜盐嗜碱球菌属。
