Lane B P, Elias J, Drummond E
J Histochem Cytochem. 1977 Jan;25(1):69-72. doi: 10.1177/25.1.833423.
The ultrastructural distribution of alpha-actinin in skeletal myocytes was studied by a sensitive method that permits localization of antigenic proteins in intact fixed cells rather than in glycerol estracted tissues or isolated cell fractions. Binding to the Z line of specific antiserum produced in rabbits immunized with highly purified alpha-actinin was demonstrated by deposition at this site of a reaction product of horseradish peroxidase that had been coupled to antigen-binding fragments of sheep anti-rabbit gamma globulin.
采用一种灵敏的方法研究了α-辅肌动蛋白在骨骼肌细胞中的超微结构分布，该方法能够定位完整固定细胞中的抗原蛋白，而非甘油抽提组织或分离的细胞组分中的抗原蛋白。用高纯度α-辅肌动蛋白免疫家兔制备的特异性抗血清与Z线的结合，通过辣根过氧化物酶反应产物沉积在该部位得以证实，辣根过氧化物酶已与羊抗兔γ球蛋白的抗原结合片段偶联。
