Arena N, Santacruz G, Corgiolu T, Alia E F, Baldus M, Alia E E
Boll Soc Ital Biol Sper. 1982 Nov 30;58(22):1485-90.
In this work we studied the alteration of Z-line alpha-actinin in striated skeletal muscle, under conditions of experimental ischemia, by immunohistochemical techniques. alpha-Actinin was extracted and purified from rabbit striated skeletal muscle and used, as an antigen, for the production of antibodies, in sheep. Electrophoresis was performed on PAA sla-gel and the Ag-Ab reaction test on agarose slab-gel (immunodiffusion). Antibodies were used for the indirect immunoperoxidase technique on semithin sections of rabbit striated skeletal muscle, included in in Araldehyde, and observed with phase-contrast microscopy. alpha-Actinin from ischemic muscle because of stenosis of the iliac artery, undergoes a progressive alteration, from the third hour of ischemia, going out of its usual sites. From the fifth to the eighth hour it gradually disappears until it leaves rare and residual points of positive reaction only in correspondence to the Z-lines.
在这项研究中，我们采用免疫组织化学技术，研究了实验性缺血条件下横纹肌中Z线α-辅肌动蛋白的变化。从兔横纹肌中提取并纯化α-辅肌动蛋白，以此作为抗原，在绵羊体内制备抗体。在聚丙烯酰胺凝胶上进行电泳，并在琼脂糖平板凝胶上进行抗原-抗体反应试验（免疫扩散）。抗体用于对包埋在戊二醛中的兔横纹肌半薄切片进行间接免疫过氧化物酶技术处理，并通过相差显微镜观察。由于髂动脉狭窄导致的缺血肌肉中的α-辅肌动蛋白，从缺血的第三个小时开始逐渐发生变化，离开其通常所在的位置。从第五到第八个小时，它逐渐消失，直到仅在对应Z线的位置留下罕见的残余阳性反应点。
