Lawrence R M, Huston A C, Hoeprich P D
J Infect Dis. 1977 Jan;135(1):117-9. doi: 10.1093/infdis/135.1.117.
Intranasal instillation of an inoculum has been suggested as a method for establishment of experimental pulmonary infections. Because we encountered untoward variation in rates and times of death with use of this method for inducing coccidioidomycosis in mice, a simple method for direct intratracheal infection was devised. With doses of 50 or 100 arthrospores of Coccidioides immitis, mortality rates were 80%-90% from 14 to 25 days after infection by the intratracheal route and 20%-60% from 12 to 24 days after infection by the intranasal route. The procedure for intratracheal infection caused only slight morbidity, and the method resulted in consistent rates of disease.
鼻内接种接种物已被提议作为建立实验性肺部感染的一种方法。由于我们在使用这种方法诱导小鼠球孢子菌病时遇到了死亡率和死亡时间的不良差异，因此设计了一种简单的直接气管内感染方法。使用50或100个粗球孢子菌关节孢子的剂量，气管内感染后14至25天的死亡率为80%-90%，鼻内感染后12至24天的死亡率为20%-60%。气管内感染的操作仅引起轻微发病，且该方法导致疾病发生率一致。
