Miura Y, Campese V, deQuattro V, Meijer D
J Lab Clin Med. 1977 Feb;89(2):421-7.
The fluorimetric method of Renzini and Valori, utilizing alumina and ion exchange chromatography, was modified to include dl-3H norepinephrine (3H-NE) for individual plasma NE recoveries and mercaptoethanol as a stabilizing agent for the fluorescent lutines. Plasma NE and epinephrine (E) were measured in hypertensive patients, and results were compared with those of the enzymatic method of Engelman in duplicate plasma specimens. The correlations of the NE and the total plasma catecholamine concentrations determined by the two methods were both r = 0.98 (p less than 0.001) and that of the E concentrations was r = 0.79 (p less than 0.01). The reproducibility of the method for measuring 10 ml. aliquots of pooled plasma yielded a standard deviation of less than 10 per cent of the mean. The fluorimetric method was more economical, less time-consuming, and as reliable as the enzymatic method for quantitating plasma catecholamines.
伦齐尼和瓦洛里的荧光法，利用氧化铝和离子交换色谱法，经过改进，加入了dl-3H去甲肾上腺素（3H-NE）用于个体血浆去甲肾上腺素回收率的测定，并加入了巯基乙醇作为荧光鲁米诺的稳定剂。对高血压患者的血浆去甲肾上腺素和肾上腺素（E）进行了测定，并将结果与恩格尔曼酶法在两份血浆标本中的测定结果进行了比较。两种方法测定的去甲肾上腺素和总血浆儿茶酚胺浓度的相关性均为r = 0.98（p小于0.001），肾上腺素浓度的相关性为r = 0.79（p小于0.01）。该方法对10毫升混合血浆等分试样进行测量的重现性，其标准偏差小于平均值的10%。荧光法更经济、耗时更少，并且在定量血浆儿茶酚胺方面与酶法一样可靠。
