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血浆儿茶酚胺测定:荧光法与放射酶法的比较

Plasma catecholamines assay: comparison between fluorimetric and radioenzymatic methods.

作者信息

Agabiti-Rosei E, Beschi M, Castellano M, Tosoni S, Signorini C

出版信息

J Clin Chem Clin Biochem. 1984 Aug;22(8):539-43. doi: 10.1515/cclm.1984.22.8.539.

DOI:10.1515/cclm.1984.22.8.539
PMID:6491616
Abstract

Plasma catecholamines were simultaneously measured in duplicate plasma samples by the fluorimetric method of Renzini et al. (1970) Clin. Chim. Acta 39, 587-594) and by the radioenzymatic method of Da Prada & Zurcher ((1976) Life Sci. 19, 1161-1174). The correlation of noradrenaline and adrenaline plasma concentrations determined by the two methods were, respectively, r = 0.95 (p less than 0.001) and r = 0.75 (p less than 0.01). The fluorimetric method was less sensitive, but more economical and less time-consuming than the radioenzymatic method. The fluorimetric method is still of value for measuring plasma catecholamines in man.

摘要

采用Renzini等人(1970年,《临床化学学报》39卷,587 - 594页)的荧光法和Da Prada与Zurcher(1976年,《生命科学》19卷,1161 - 1174页)的放射酶法,对两份血浆样本同时进行血浆儿茶酚胺测定。两种方法测定的去甲肾上腺素和肾上腺素血浆浓度的相关性分别为:r = 0.95(p < 0.001)和r = 0.75(p < 0.01)。荧光法的灵敏度较低,但比放射酶法更经济且耗时更少。荧光法在测定人体血浆儿茶酚胺方面仍有价值。

相似文献

1
Plasma catecholamines assay: comparison between fluorimetric and radioenzymatic methods.血浆儿茶酚胺测定:荧光法与放射酶法的比较
J Clin Chem Clin Biochem. 1984 Aug;22(8):539-43. doi: 10.1515/cclm.1984.22.8.539.
2
Plasma catecholamines via an improved fluorimetric assay: comparison with an enzymatic method.通过改进的荧光测定法检测血浆儿茶酚胺:与酶法的比较。
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Inter-laboratory comparison of plasma catecholamine determinations using several different assays.使用几种不同检测方法对血浆儿茶酚胺测定进行实验室间比对。
Acta Physiol Scand Suppl. 1984;527:43-54.
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Single-isotope enzymatic derivative method for measuring catecholamines in human plasma.用于测量人血浆中儿茶酚胺的单同位素酶衍生法。
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Radioenzymatic assay of plasma adrenaline and noradrenaline: evidence for a catechol-O-methyltransferase (COMT) inhibiting factor associated with essential hypertension.血浆肾上腺素和去甲肾上腺素的放射酶法测定:与原发性高血压相关的儿茶酚-O-甲基转移酶(COMT)抑制因子的证据。
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An improved method for the fluorometric determination of tissue catecholamines.一种改进的荧光法测定组织儿茶酚胺的方法。
Neurochem Res. 1986 May;11(5):753-8. doi: 10.1007/BF00965343.