Kaĭdashev I P, Katrushov A V, Mishchenko V P
Fiziol Zh (1978). 1993 Jan-Feb;39(1):19-23.
The treatment of erythrocytes with different enzymes (neuraminidase, trypsin and papain) has been studied for its effect on the lipid peroxidation in their membranes, activity of antioxidant enzymes and on the ability of such erythrocytes to interact with neutrophilic leucocytes. It is shown that desialization of the membrane erythrocyte structures causes the intensification of lipid peroxidation in the membranes, decrease of their stability. Simultaneously, the interaction of neutrophils increases. The treatment with proteases causes the opposite changes. It is shown possible to split out the end molecules of sialic acids with exposure of the subterminal residues of B-galactose as a result of the effect of active oxygen forms. An assumption is advanced concerning the functional conjugation of the free-radical oxidation and proteolysis in provision of stability of the cell membranes.
研究了用不同酶（神经氨酸酶、胰蛋白酶和木瓜蛋白酶）处理红细胞对其膜脂质过氧化、抗氧化酶活性以及此类红细胞与嗜中性白细胞相互作用能力的影响。结果表明，红细胞膜结构的去唾液酸化会导致膜中脂质过氧化加剧，稳定性降低。同时，中性粒细胞的相互作用增加。用蛋白酶处理则会产生相反的变化。结果表明，由于活性氧形式的作用，有可能裂解唾液酸的末端分子，使β-半乳糖的亚末端残基暴露出来。提出了关于自由基氧化与蛋白水解在维持细胞膜稳定性方面功能共轭的假设。
