从携带伤寒沙门氏菌来源的小型多拷贝Hsd质粒的大肠杆菌K-12中分离并鉴定StyD4I限制性内切酶，它是ScrFI的新裂变体。
Isolation and characterization of the StyD4I restriction endonuclease, a neoschizomer of ScrFI, from Escherichia coli K-12 carrying a small multicopy Hsd plasmid of Salmonella typhi origin.
作者信息
Miyahara M, Mise K
机构信息
Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan.
出版信息
Gene. 1993 Jul 15;129(1):83-6. doi: 10.1016/0378-1119(93)90699-4.
A restriction endonuclease, designated StyD4I, a neoschizomer of ScrFI, has been isolated from Escherichia coli K-12 carrying a small multicopy host specificity for DNA (Hsd) plasmid of Salmonella typhi D4 origin. In the presence of 10 mM Mg2+, StyD4I cleaves the sequence 5'-/CCNGG-3' and generates a 5-nucleotide cohesive end. StyD4I should be useful for recombinant DNA technology, because of the stability and ease in handling the producer cells.
一种名为StyD4I的限制性内切酶，是ScrFI的新裂变体，已从携带伤寒沙门氏菌D4来源的小多拷贝宿主特异性DNA（Hsd）质粒的大肠杆菌K-12中分离出来。在10 mM Mg2+存在的情况下，StyD4I切割序列5'-/CCNGG-3'并产生一个5个核苷酸的粘性末端。由于生产细胞的稳定性和易于处理，StyD4I在重组DNA技术中应该会很有用。
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