从携带hsd⁺微型质粒的大肠杆菌中纯化一种新型限制性内切酶StyI。
Purification of a new restriction endonuclease, StyI, from Escherichia coli carrying the hsd+ miniplasmid.
作者信息
Mise K, Nakajima K
出版信息
Gene. 1985;33(3):357-61. doi: 10.1016/0378-1119(85)90244-6.
A new restriction endonuclease, StyI, free of contaminating nuclease activities, has been isolated from Escherichia coli carrying the hsd+ miniplasmid of Salmonella typhi origin. In the presence of 10 mM Mg2+, it recognizes and cleaves a hexanucleotide sequence of 5'-C decreases C(AT)(AT)GG. The advantages of the StyI endonuclease include its stability, high yield (more than 2 X 10(3) units/g of wet cells), easy handling of producer cells, and the ability to recognize new sequences, CCAAGG and CCTTGG.
一种新的限制性内切酶StyI已从携带伤寒沙门氏菌来源的hsd + 微型质粒的大肠杆菌中分离出来，该酶无核酸酶污染活性。在10 mM Mg2+存在的情况下，它识别并切割5'-C↓C(AT)(AT)GG的六核苷酸序列。StyI内切酶的优点包括其稳定性、高产量（每克湿细胞超过2×10³单位）、生产细胞易于处理以及识别新序列CCAAGG和CCTTGG的能力。
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