Mori K, Kaido M, Omura M, Fujishiro K, Inoue N, Koide O
Department of Environmental Toxicology, University of Occupational and Environmental Health, Kitakyushu.
Fukuoka Igaku Zasshi. 1993 Jun;84(6):322-9.
In the present study, we tried to use the hemiorchiectomy method to examine a reversibility of the testicular damage induced by ethylene oxide (EtO). Wistar male rats were exposed to EtO at a concentration of 500 ppm, 6 hours a day, 3 days a week for 8 weeks. Thereafter we evaluated the reversibility after 13-week recovery period. From the result about a propriety of the hemiorchiecyomy method for recovery study, it was suggested that hemiorchiectomy did not affect remaining testis and epididymis after the recovery period and there was no laterality about the testicular damage just after the exposure period. Therefore we concluded that the hemiorchiectomy method could be used for recovery study for EtO-induced testicular damage. The reversibility was evaluated by comparing the damages of hemiorchiectomized testis just after the exposure period and those of remaining testis removed after the recovery period in the same rat. The result indicated that the reversibility of EtO-induced testicular damage may depend on a degree of damage just after the exposure.
在本研究中，我们尝试使用半侧睾丸切除术方法来检测环氧乙烷（EtO）诱导的睾丸损伤的可逆性。将Wistar雄性大鼠暴露于浓度为500 ppm的EtO中，每天6小时，每周3天，持续8周。此后，我们评估了13周恢复期后的可逆性。从半侧睾丸切除术方法用于恢复研究的适宜性结果来看，表明半侧睾丸切除术在恢复期后不影响剩余的睾丸和附睾，并且在暴露期刚结束时睾丸损伤不存在左右侧差异。因此，我们得出结论，半侧睾丸切除术方法可用于EtO诱导的睾丸损伤的恢复研究。通过比较同一只大鼠在暴露期刚结束时半侧切除睾丸的损伤与恢复期后切除剩余睾丸的损伤来评估可逆性。结果表明，EtO诱导的睾丸损伤的可逆性可能取决于暴露期刚结束时的损伤程度。
