Zhong X, Zhang L, Wang Y
Division of Occupational Medicine Jinshan Hospital, Shanghai Medical University.
Zhonghua Yu Fang Yi Xue Za Zhi. 1997 May;31(3):160-2.
Male Wistar rats were exposed to ethylene Oxide (Eto) via inhalation at a concentration of 751.7 mg/m3 two hours a day and six days a week for nine consecutive weeks. Sensitivity test of restriction enzymes Mbo I, Sau3A I, Hpa II and Msp I and Southern blot were conducted to study the effects of ethylene oxide on activity of major intracellular modification enzyme, DNA methylase. Results showed that DNA in liver, kidney and testis of rats were completely enzymolysed and only one blot was seen after being treated with Hind III, Mbo I and Sau3A I, and incompletely enzymolysed and multiple blots were seen after being treated with Hpa II and Msp I. There were no significant differences in electrophoresis pattern, number of blots and intensity of radioautograph between the exposed animals and controls. It indicated that no effects of Eto on activity of DNA methylase in the liver, kidney and testis of rats was found, and degree of methylation in the testis was much less than that in the liver and kidney.
将雄性Wistar大鼠暴露于环氧乙烷（EtO）中，以751.7毫克/立方米的浓度，每天吸入2小时，每周6天，连续9周。进行了限制性内切酶Mbo I、Sau3A I、Hpa II和Msp I的敏感性测试以及Southern印迹分析，以研究环氧乙烷对主要细胞内修饰酶DNA甲基化酶活性的影响。结果显示，用Hind III、Mbo I和Sau3A I处理后，大鼠肝脏、肾脏和睾丸中的DNA被完全酶解，仅见一条印迹；而用Hpa II和Msp I处理后，DNA未被完全酶解，可见多条印迹。暴露组动物与对照组在电泳图谱、印迹数量和放射自显影片强度方面均无显著差异。这表明未发现环氧乙烷对大鼠肝脏、肾脏和睾丸中DNA甲基化酶活性有影响，且睾丸中的甲基化程度远低于肝脏和肾脏。
