甲萘醌（维生素K2）的生物合成：大肠杆菌menC基因的克隆、核苷酸序列及表达
Menaquinone (vitamin K2) biosynthesis: cloning, nucleotide sequence, and expression of the menC gene from Escherichia coli.
作者信息
Sharma V, Meganathan R, Hudspeth M E
机构信息
Department of Biological Sciences, Plant Molecular Biology Center, Northern Illinois University, DeKalb 60115.
出版信息
J Bacteriol. 1993 Aug;175(15):4917-21. doi: 10.1128/jb.175.15.4917-4921.1993.
Abstract
The benzenoid aromatic compound o-succinylbenzoic acid is formed by dehydration of the prearomatic compound 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid by the enzyme o-succinylbenzoate synthase, encoded by the menC gene. A 1.3-kb PstI-PvuII fragment was found to complement the menC mutation. The complete nucleotide sequence of this fragment revealed a single open reading frame of 954 bp capable of encoding a 35-kDa protein. A consensus sequence for a ribosomal binding site but no promoter consensus sequences were found. However, the first base of the initiating codon of this open reading frame overlaps the upstream menB gene termination codon, suggesting an operon-like organization for these genes. Consistent with this suggestion, the menB promoter can initiate transcription of the menC gene.
摘要
苯环型芳香化合物邻琥珀酰苯甲酸是由前芳香化合物2-琥珀酰-6-羟基-2,4-环己二烯-1-羧酸通过由menC基因编码的邻琥珀酰苯甲酸合酶脱水形成的。发现一个1.3 kb的PstI - PvuII片段可弥补menC突变。该片段的完整核苷酸序列揭示了一个954 bp的单一开放阅读框，能够编码一种35 kDa的蛋白质。发现了核糖体结合位点的共有序列，但未发现启动子共有序列。然而，这个开放阅读框起始密码子的第一个碱基与上游menB基因的终止密码子重叠，表明这些基因具有操纵子样的组织形式。与这一推测一致的是，menB启动子可以启动menC基因的转录。
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