Choi A Y, Cahill A L, Perry B D, Perlman R L
Department of Pharmacological Science, University of Chicago, Illinois 60637.
J Neurochem. 1993 Aug;61(2):541-9. doi: 10.1111/j.1471-4159.1993.tb02157.x.
Chromaffin cells have H1 histamine receptors. Histamine, acting at these receptors, increases the metabolism of inositol-containing phospholipids and stimulates catecholamine secretion from chromaffin cells. We have investigated the effects of histamine and other agents on the accumulation of inositol monophosphate (InsP1) and catecholamine secretion in purified cultures of norepinephrine-containing and epinephrine-containing bovine chromaffin cells. Histamine-stimulated InsP1 accumulation in epinephrine cells was three times greater than that in norepinephrine cells. In contrast, bradykinin caused roughly equivalent increases in InsP1 accumulation in the two chromaffin cell subtypes. Histamine-stimulated catecholamine secretion was also greater in epinephrine cells than in norepinephrine cells, whereas high K+, bradykinin, phorbol 12,13-dibutyrate, and angiotensin II all caused greater secretion from norepinephrine cells than from epinephrine cells. The density of H1 receptors in epinephrine cells was approximately three times greater than that in norepinephrine cells. The greater density of H1 receptors on epinephrine cells may account for the greater effects of histamine on InsP1 accumulation and catecholamine secretion in these cells.
嗜铬细胞具有H1组胺受体。组胺作用于这些受体，可增加含肌醇磷脂的代谢，并刺激嗜铬细胞分泌儿茶酚胺。我们研究了组胺和其他药物对纯化培养的含去甲肾上腺素和含肾上腺素的牛嗜铬细胞中肌醇一磷酸（InsP1）积累和儿茶酚胺分泌的影响。组胺刺激肾上腺素细胞中InsP1的积累比去甲肾上腺素细胞中的大三倍。相比之下，缓激肽在两种嗜铬细胞亚型中引起的InsP1积累增加大致相当。组胺刺激的儿茶酚胺分泌在肾上腺素细胞中也比在去甲肾上腺素细胞中更大，而高钾、缓激肽、佛波酯12,13 - 二丁酸酯和血管紧张素II在去甲肾上腺素细胞中引起的分泌都比在肾上腺素细胞中更大。肾上腺素细胞中H1受体的密度大约比去甲肾上腺素细胞中的大三倍。肾上腺素细胞上H1受体密度更高可能解释了组胺对这些细胞中InsP1积累和儿茶酚胺分泌的更大影响。
