Garris P A, Collins L B, Jones S R, Wightman R M
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
J Neurochem. 1993 Aug;61(2):637-47. doi: 10.1111/j.1471-4159.1993.tb02168.x.
The measurement of evoked extracellular dopamine in the medial prefrontal cortex by using fast-scan cyclic voltammetry with carbon-fiber microelectrodes was established and release characteristics of mesoprefrontal dopamine neurons were examined in vivo in anesthetized rats. Despite the sparse dopaminergic innervation and the presence of more dense noradrenergic and serotonergic innervations overall in the medial prefrontal cortex, the measurement of extracellular dopamine was achieved by selective recording in dopamine-rich terminal fields and selective activation of ascending dopamine neurons. This was confirmed by electrochemical, pharmacological, and anatomical evidence. An increased release capacity for mesoprefrontal dopamine neurons was also demonstrated by the slower decay of the evoked dopamine response after inhibition of catecholamine synthesis and the maintenance of the evoked dopamine response at higher levels in the medial prefrontal cortex compared with the striatum during supraphysiological stimulation.
利用碳纤维微电极通过快速扫描循环伏安法测定内侧前额叶皮质中诱发的细胞外多巴胺，并在麻醉大鼠体内研究了内侧前额叶多巴胺能神经元的释放特性。尽管内侧前额叶皮质中多巴胺能神经支配稀疏，且整体上去甲肾上腺素能和5-羟色胺能神经支配更为密集，但通过在富含多巴胺的终末区域进行选择性记录和对上行多巴胺能神经元进行选择性激活，实现了细胞外多巴胺的测量。这一点得到了电化学、药理学和解剖学证据的证实。与纹状体相比，在超生理刺激期间，抑制儿茶酚胺合成后诱发的多巴胺反应衰减较慢，且内侧前额叶皮质中诱发的多巴胺反应维持在较高水平，这也证明了内侧前额叶多巴胺能神经元的释放能力增强。
