Knapka J J, Smith K P, Judge F J
J Nutr. 1977 Jan;107(1):61-71. doi: 10.1093/jn/107.1.61.
Diets made from natural ingredients were fed to four inbred strains of mice (BALB/cAnN, C3H/HeN, C57BL/6N and DBA/2N) to study the effects of different concentrations of dietary crude protein, 18% and 24% with crude fat concentrations of 4%, 8%, and 12% on reproduction and weanling growth. The parameters measured included the number of litters and pups born, the number of litters and pups weaned, weanling mortality and weanling weight. Neither crude protein nor crude fat concentrations had significant effects on any of the reproductive parameters tested. However, a significant fat x protein interaction was observed for reproduction. These results indicate that the absolute concentrations of crude protein and crude fat in diets for inbred mouse production are not as important as the ratio of these two nutrients. There was also a differential response in reproduction among the strains due to the level of fat which indicates different dietary requirements for fat among these four inbred strains for maximum reproduction. In contrast to the results for reproduction, crude protein and crude fat independently had significant effects on weanling weight, but there was no effect due to the ratio of the nutrients. There was a significant increase in the weanling growth rate for all four strains as the dietary fat level increased, but a decrease in growth rate as the protein level increased. The reduced growth rate due to the increased protein was not of the same magnitude for all four strains which indicates specific protein requirements among the strains for weanling growth.
用天然成分制成的日粮饲喂四种近交系小鼠（BALB/cAnN、C3H/HeN、C57BL/6N和DBA/2N），以研究不同浓度的日粮粗蛋白（18%和24%）和粗脂肪浓度（4%、8%和12%）对繁殖和断奶仔鼠生长的影响。所测量的参数包括产仔数和出生仔鼠数、断奶仔鼠数和断奶仔鼠数、断奶死亡率和断奶体重。粗蛋白和粗脂肪浓度对所测试的任何繁殖参数均无显著影响。然而，在繁殖方面观察到显著的脂肪×蛋白交互作用。这些结果表明，用于近交系小鼠生产的日粮中粗蛋白和粗脂肪的绝对浓度不如这两种营养素的比例重要。由于脂肪水平的不同，各品系在繁殖方面也有不同的反应，这表明这四种近交系小鼠为实现最大繁殖对脂肪的日粮需求不同。与繁殖结果相反，粗蛋白和粗脂肪分别对断奶体重有显著影响，但营养素比例没有影响。随着日粮脂肪水平的增加，所有四个品系的断奶生长率均显著提高，但随着蛋白水平的增加，生长率下降。由于蛋白增加导致的生长率下降在所有四个品系中幅度不同，这表明各品系在断奶生长方面对蛋白有特定需求。
