Taber S W, Wieman T J, Fingar V H
University of Louisville, Department of Surgery, Brown Cancer Center, KY 40292.
Photochem Photobiol. 1993 May;57(5):856-61. doi: 10.1111/j.1751-1097.1993.tb09224.x.
The effects of aspirin (acetylsalicylic acid: ASA) on vessel behavior and tumor response were measured during and after photodynamic therapy (PDT). Changes to vessel constriction, macromolecular leakage, tumor interstitial pressure, and tumor response were examined. Animals were randomly placed into treatment groups and injected with 0-25 mg/kg Photofrin and given 0 or 135 J/cm2 light treatment. The light treatment was standardized to 75 mW/cm2 at 630 nm over a 30 min treatment interval (135 J/cm2). The treatment groups were further subdivided to receive Photofrin alone or Photofrin plus 100 mg/kg ASA. A cremaster muscle model in Sprague-Dawley rats was used to directly observe microvascular response and changes in vessel permeability to macromolecules. A tumor interstitial pressure model was designed to measure pressure changes in a chondrosarcoma tumor over time. This model indirectly measures macromolecular leakage, among other factors, in the tumor tissue. Groups of 10-20 rats were implanted subcutaneously with chondrosarcoma and were subjected to PDT to assess tumor response to the various treatments. Statistically significant differences in vessel leakage and changes in interstitial pressure were observed between animals given ASA plus PDT as compared to animals given PDT alone. The administration of ASA significantly inhibited venule leakage of albumin and reduced increases in interstitial pressure after treatment. The use of ASA had no effect on vessel constriction or tumor response after PDT. These findings suggest that the increases in vessel permeability observed during and after PDT, using Photofrin, do not significantly contribute to tumor response.
在光动力疗法（PDT）期间及之后，测量了阿司匹林（乙酰水杨酸：ASA）对血管行为和肿瘤反应的影响。研究了血管收缩、大分子渗漏、肿瘤间质压力和肿瘤反应的变化。将动物随机分为治疗组，注射0 - 25 mg/kg的卟吩姆钠，并给予0或135 J/cm²的光治疗。光治疗在630 nm波长下以75 mW/cm²的功率标准化进行30分钟（135 J/cm²）。治疗组进一步细分，分别接受单独的卟吩姆钠或卟吩姆钠加100 mg/kg ASA。使用Sprague-Dawley大鼠的提睾肌模型直接观察微血管反应和血管对大分子通透性的变化。设计了一个肿瘤间质压力模型来测量软骨肉瘤肿瘤随时间的压力变化。该模型间接测量肿瘤组织中的大分子渗漏等因素。将10 - 20只大鼠皮下植入软骨肉瘤，并进行PDT以评估肿瘤对各种治疗的反应。与单独接受PDT的动物相比，接受ASA加PDT的动物在血管渗漏和间质压力变化方面存在统计学上的显著差异。ASA的给药显著抑制了白蛋白的小静脉渗漏，并减少了治疗后间质压力的升高。在PDT后使用ASA对血管收缩或肿瘤反应没有影响。这些发现表明，在使用卟吩姆钠的PDT期间及之后观察到的血管通透性增加对肿瘤反应没有显著贡献。
