Hill B D, Dawson A M, Blewett D A
Moredun Research Institute, Edinburgh.
Res Vet Sci. 1993 May;54(3):356-60. doi: 10.1016/0034-5288(93)90135-3.
Sporozoites of Cryptosporidium parvum were incubated in 1:10 dilutions of immune or non-immune, heat-inactivated lamb serum specimens or serum fractions. The infectivity of treated sporozoites was assessed by inoculating them, per rectum, into five-day-old rats followed by histological examination of their intestines at either three or five days after infection. The infectivity of sporozoites treated with heat-inactivated whole sera was greatly reduced. This neutralisation had both specific and non-specific components. The former was associated with the IgG fraction of hyperimmune serum raised against sporozoites and the latter with a heat-stable, non-dialysable component present in both IgG-depleted hyperimmune serum and uninfected gnotobiotic serum.
微小隐孢子虫的子孢子在免疫或非免疫、热灭活的羔羊血清标本或血清组分的1:10稀释液中孵育。通过经直肠将处理过的子孢子接种到5日龄大鼠体内，然后在感染后3天或5天对其肠道进行组织学检查，评估处理过的子孢子的感染性。用热灭活的全血清处理的子孢子的感染性大大降低。这种中和作用既有特异性成分，也有非特异性成分。前者与针对子孢子产生的超免疫血清的IgG组分有关，后者与IgG缺失的超免疫血清和未感染的悉生动物血清中存在的一种热稳定、不可透析的成分有关。
