Ortega-Mora L M, Troncoso J M, Rojo-Vazquez F A, Gomez-Bautista M
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Spain.
Vet Parasitol. 1994 May;53(1-2):159-66. doi: 10.1016/0304-4017(94)90028-0.
The appearance, persistence and eventual decline of IgG, IgM and IgA antibodies recognizing Cryptosporidium parvum antigens were studied in naturally infected lambs using a Western blot technique, and the results compared with those obtained using sera from immunized lambs. There was an intense recognition of some low molecular weight proteins (15-17 kDa by IgG and IgA; 28-30 kDa by IgA, IgM and IgG) during the infection and early post-infection period. These peptides were not recognized after Days 45-60 of life. Some high molecular weight antigens (94 kDa) were weakly recognized on Day 15 but more intensely recognized from Day 30 onwards, persisting until at least Day 90. Antibody recognition of these C. parvum proteins could be an indicator of recent or past exposure to the parasite.
利用蛋白质免疫印迹技术，研究了自然感染隐孢子虫的羔羊体内识别微小隐孢子虫抗原的IgG、IgM和IgA抗体的出现、持续存在及最终下降情况，并将结果与免疫羔羊血清的检测结果进行了比较。在感染期间和感染后早期，一些低分子量蛋白质（IgG和IgA识别的分子量为15 - 17 kDa；IgA、IgM和IgG识别的分子量为28 - 30 kDa）被强烈识别。这些肽在羔羊出生后45 - 60天之后未被识别。一些高分子量抗原（94 kDa）在第15天时被弱识别，但从第30天起被更强烈地识别，至少持续到第90天。对这些微小隐孢子虫蛋白质的抗体识别可能是近期或过去接触该寄生虫的一个指标。
