Kato T, Katagiri T, Shikama Y, Kurita K, Toyoshima I, Hirano A, Wada M, Sasaki H
Third Department of Internal Medicine, Yamagata University School of Medicine, Japan.
Acta Neuropathol. 1993;85(6):663-5. doi: 10.1007/BF00334678.
The spinal cords from eight autopsy cases of sporadic motor neuron disease (MND) and two control cases were immunohistochemically examined using antibodies directed to neurofilament proteins (anti-Nf) and to heparan sulfate (HepSS-1). Variable numbers of spheroids were observed in the anterior horns in the MND cases. In one case of MND, one third to half of the remaining anterior horn cells contained conglomerate inclusions in their perikarya. These pathological structures were not encountered in the control cases. The immunohistochemical study revealed that both anti-Nf and HepSS-1 intensely labelled all spheroids and conglomerate inclusions in the MND cases. The colocalization of heparan sulfate with neurofilamentous accumulation suggests that heparan sulfate is required for the aggregation of neurofilaments, resulting in the formation of spheroids and conglomerate inclusions in MND.
使用针对神经丝蛋白（抗Nf）和硫酸乙酰肝素（HepSS-1）的抗体，对8例散发性运动神经元病（MND）尸检病例和2例对照病例的脊髓进行了免疫组织化学检查。在MND病例的前角观察到数量不等的球体。在1例MND病例中，剩余前角细胞的三分之一至一半在其核周含有聚集性包涵体。对照病例中未发现这些病理结构。免疫组织化学研究表明，在MND病例中，抗Nf和HepSS-1均强烈标记所有球体和聚集性包涵体。硫酸乙酰肝素与神经丝聚集的共定位表明，硫酸乙酰肝素是神经丝聚集所必需的，导致MND中球体和聚集性包涵体的形成。
